An Enzymatic Method for Isolating Cardiac Myocytes From Heart of Guinea-Pig Used for Single-Cell and Patch-Clamp Studies
| dc.contributor.author | Meral, Ismail | |
| dc.date.accessioned | 2025-05-10T16:44:15Z | |
| dc.date.available | 2025-05-10T16:44:15Z | |
| dc.date.issued | 1997 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | Van Yüzüncü Yil Üni̇versi̇tesi̇ | en_US |
| dc.description.abstract | A method for isolating cardiac myocytes from the heart of guinea-pig is described. This method consists of perfusion of the heart with a low Ca{2+} medium and subsequent dispersion of cells with collagenase and proteolytic enzyme. More than 80% rod-shoped, viable and quiescent cells which are suitable for electrophysiological and pharmacological experiments are obtained by this method. | en_US |
| dc.identifier.endpage | 567 | en_US |
| dc.identifier.issn | 1300-0144 | |
| dc.identifier.issn | 1303-6165 | |
| dc.identifier.issue | 6 | en_US |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.startpage | 565 | en_US |
| dc.identifier.trdizinid | 43269 | |
| dc.identifier.uri | https://search.trdizin.gov.tr/en/yayin/detay/43269/an-enzymatic-method-for-isolating-cardiac-myocytes-from-heart-of-guinea-pig-used-for-single-cell-and-patch-clamp-studies | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/483 | |
| dc.identifier.volume | 27 | en_US |
| dc.identifier.wosquality | Q3 | |
| dc.institutionauthor | Meral, Ismail | |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Turkish Journal of Medical Sciences | en_US |
| dc.relation.publicationcategory | Diğer | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Biyoloji | en_US |
| dc.subject | Biyokimya Ve Moleküler Biyoloji | en_US |
| dc.subject | Hücre Biyolojisi | en_US |
| dc.title | An Enzymatic Method for Isolating Cardiac Myocytes From Heart of Guinea-Pig Used for Single-Cell and Patch-Clamp Studies | en_US |
| dc.type | Other | en_US |