Effects of Colchicine and High Temperature Treatments on Isolated Microspore Culture in Various Cabbage (Brassica Oleraceae) Types

Abstract

The effect of colchicine with heat shock treatments on the microspore embryogenesis were evaluated in white head cabbage (B. oleracea var. capitata subvar. alba), kale (B. oleracea var. acephala) and ornamental kale (B. oleracea var. acephala). 2 different colchicine doses (50 & 100 mg/L) applied to the isolated microspores in order to stimulate the embryo induction for 15 h. After colchicine treatments microspores were exposed to 32 degrees C and 35 degrees C temperature in NLN-13 medium (40,000 microspore/mL) for 48 h. According to the 12 day and 19 day embryo yields after planting 50 mg/L colchicine treatment was found more effective. 32 degrees C+50 mg/L colchicine treatment (5.3 embryo/petri) in white head cabbage (Yalova-1) and 35 degrees C+50 mg/L colchicine treatment (9.4 embryo/petri) in ornamental kale were found effective treatments, respectively. White head cabbage (Er is genotype) and kale did not give positive respond to the colchicine treatments. (C) 2011 Friends Science Publishers