Development of Potentiometric Sensor for Determination of Isoprenaline in Pharmaceutical Drug

Abstract

In this study, a potentiometric ion-selective electrode (ISE) was developed for the determination of isoprenaline, a pharmaceutical active substance. For this purpose, initially, IP-PM, IP-TCA, IP-TPB, IP-REY, IP-PTA ion pairs were synthesized. The synthesized ion pairs were used as the ionophore in the structure of the electrode membrane. In order to ensure membrane optimization, PVC membrane ion selective electrodes were produced in various compositions using the synthesized ion pair and the potentiometric performance characteristics of these electrodes were investigated. It was determined that the best potentiometric performance characteristics were obtained with the PVC membrane electrode in the composition of 3.0% isoprenaline-tetrafenylborate ion pair, %64.0 nitrophenyloctylether, 32.0% polyvinylchloride and %1.0 Tetrakis (4-chlorophenyl) boron potassium compound. The linear operating range of this electrode is 5.0x 10-6 M-1.0x 10-1 M and the slope at the 10-fold concentration change is 45.3 mV; determination limit, 5.0x10-6 M, pH working range, 2.6-3.6 and 5.7-7.9; response time < 5s. The electrode exhibited a reproducible potentiometric response. Isoprenaline content was determined in drug by using the isoprenaline selective electrode. © 2020, TUBITAK. All rights reserved.