Electrochemical Determination of Anti-Cancer Drug Dabrafenib With High Sensitivity Using Multi-Walled Carbon Nano Tubes Modified Glassy Carbon Electrode

Abstract

Dabrafenib (DAB) is an organofluor compound and a protein kinase inhibitor used as the mesylate salt in the treatment of metastatic melanoma. For the first time, the electrochemical investigation of the anticancer drug DAB in human serum was conducted using a glassy carbon electrode modified with multi-walled carbon nanotubes (MWCNTs), employing cyclic voltammetry and square wave voltammetry (SWV) techniques. Electrochemical impedance spectroscopy, cyclic voltammetry, and scanning electron microscopy (SEM) techniques were utilized to analyze the surface morphology and structure of the MWCNT/GC electrode. In the proposed method using optimized experimental conditions, two different linearities were obtained for DAB in the concentration range of 0.04-0.8 mu M and 1.0-10.0 mu M. The LOD value obtained is 0.014 mu M. In this study, the resulting electrochemical sensor was applied for the first time to investigate the electrochemical behavior of DAB with high sensitivity and reproducibility, as well as the possible electrochemical oxidation mechanism. Dabrafenib, a organofluor compound and a protein kinase inhibitor, was electrochemically analyzed for the first time on a MWCNT-modified GCE, proposing an oxidation mechanism. Surface morphology and structure were examined using electrochemical impedance spectroscopy, cyclic voltammetry, and scanning electron microscopy. The electrode reaction was irreversible and diffusion-controlled, accompanied by a -2e-/2H+ oxidation reaction. Under optimized experimental conditions, two different linear ranges were obtained for DAB in 0.2 M H2SO4 solution, ranging from 0.04 to 0.8 mu M and 1.0 to 10.0 mu M, with a LOD value of 0.014 mu M. This developed method was applied to biological samples. image