Detection of Cryptosporidium Spp. in Calves Through Nested Pcr and Kinyoun's Acid-Fast Methods in Igdir, Turkey

Abstract

The present study was conducted to determine the prevalence of cryptosporidiosis by using Kinyoun's acid-fast and Nested PCR methods in 0-01 month aged calves in Igdir Province, Turkey. A total of 100 calves were used in this study. The calves belonging to the Igdir Provincial Directorate of Agriculture and Forestry, were diagnosed to have diarrhea on the basis of their clinical and anamnesis. In the microscopic examinations of 100 samples, 34 (34%) Cryptosporidium spp. were detected in Kinyoun's acid-fast whereas in the Nested PCR method it was found 38 (38%). Accordingly, attention was drawn to the necessity of complying with protective measures due to its zoo notic importance.