Optimization of Uricase Enzyme Produced by Moderately Haloalkalitolerant Halomonas Sp. F1 Isolate Characterized and Isolated From Lake Van, Turkey

Abstract

In this study, isolates were isolated from Lake Van, a soda lake, and tested for their ability to produce the enzyme uricase. M9 minimal salt medium was used for the primary screening studies. The activity of the enzyme was then determined in different media compositions by analyzing the activity of the crude enzyme solution. In the presence of yeast extract (436.58 U/ml), used as a source of organic nitrogen, the highest activity was determined. In addition, phenotypic and molecular identification of the uricase-positive isolate was performed. Within the scope of molecular identification, the 16S ribosomal RNA region was amplified by polymerase chain reaction using the appropriate universal primers. This isolate was determined by the phylogenetic analysis of the 16S rRNA gene, based on the neighboring joining method, in the genus Halomonas.