Determination of Vitamin E Levels in Walnuts (Juglans Regia L.) Grown in the Lake Van Basin by Hplc

Abstract

Aim of study: The present study describes determination of vitamin E levels in Walnuts (Juglans regia L.) Grown in the Lake Van basin. Area of study: Adilcevaz, Bahcesaray, Catak, Edremit, Gevas regions in the Lake Van basin and in the center of Van. Material and methods: Walnuts were collected regions and stored in a deep freezer for analysis. On the day of analysis, 10 pieces of fresh and dried walnut samples belonging to each region were taken and broken, and a homogeneous mixture was obtained by pulverizing them and determined by HPLC. Main results: The amounts of alpha-Tocopherol of the samples in fresh walnuts were found to be 2.36 +/- 0.01 mu g/g, 5.01 +/- 0.31 mu g/g, 6.23 +/- 0.15 mu g/g, 7.20 +/- 0.28 mu g/g, 3.99 +/- 0.46 mu g/g, 5.05 +/- 0.30 mu g/g, respectively, and the amounts of gamma-Tocopherol were found to be 23.80 +/- 0.52 mu g/g, 16.54 +/- 1.08 mu g/g, 14.82 +/- 0.46 mu g/g, 17.34 +/- 0.78 mu g/g, 11.91 +/- 0.40 mu g/g, 15.57 +/- 0.90 mu g/g, respectively, according to the regions. The amounts of alpha-Tocopherol in dried walnuts were found to be 5.13 +/- 0.26 mu g/g, 6.84 +/- 0.31 mu g/g, 7.58 +/- 0.40 mu g/g, 10.49 +/- 0.51 mu g/g, 8.65 +/- 0.34 mu g/g, 5.82 +/- 0.19 mu g/g, respectively, and the amounts of.-Tocopherol were found to be 33.10 +/- 1.65 mu g/g, 21.97 +/- 0.87 mu g/g, 20.98 +/- 0.83 mu g/g, 29.19 +/- 0.80 mu g/g, 21.09 +/- 0.24 mu g/g, 18.41 +/- 0.82 mu g/g, respectively, according to the regions. Highlights: The results obtained in the study are considered, it is observed that the walnuts grown in Adilcevaz, Bahcesaray, Catak, Edremit, Gevas and Van-Center regions are of high quality.