In Vitro Evaluation of the Effect of Glutathione on Caspase System \rand Oxidative Dna Damage In High Glucose Condition

Abstract

This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent \rapoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated \rby regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 \rmM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and \ranalyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using \rcommercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. \rIn GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which \ris the indicator of oxidative DNA damage.