In Vitro Evaluation of the Effect of Glutathione on Caspase System \rand Oxidative Dna Damage In High Glucose Condition
| dc.contributor.author | Yüksek, Veysel | |
| dc.contributor.author | Çetın, Sedat | |
| dc.contributor.author | Usta, Ayşe | |
| dc.contributor.author | Yaycı, Gülay | |
| dc.contributor.author | Dede, Semiha | |
| dc.date.accessioned | 2025-05-10T17:53:31Z | |
| dc.date.available | 2025-05-10T17:53:31Z | |
| dc.date.issued | 2021 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | Van Yüzüncü Yil Üni̇versi̇tesi̇,Van Yüzüncü Yil Üni̇versi̇tesi̇,Van Yüzüncü Yil Üni̇versi̇tesi̇,Sağlik Bi̇li̇mleri̇ Üni̇versi̇tesi̇,Van Yüzüncü Yil Üni̇versi̇tesi̇ | en_US |
| dc.description.abstract | This study was planned to investigate the effects of glutathione, known to be a strong antioxidant, on caspase-dependent \rapoptosis and oxidative DNA damage in the kidney cells (BHK-21) exposed to high glucose. BHK-21 cell line was cultivated \rby regular passages in vitro conditions. Study groups were set up as control, study and its combinations groups ((Glucose; (285 \rmM, HG), glutathione (250 µM)). After 24 hours of incubation, trypsinized cells were disrupted by freeze-thaw method and \ranalyte was prepared. Caspase 3, 8, 9, M30 and 8-OHdG (oxidative DNA damage marker) levels were determined using \rcommercial ELISA kits. M30, caspase 3, 8 and 9 which are the parameters of apoptosis, were found highest in the HG group. \rIn GSH-treated groups, these parameters decreased slightly. There was no significant difference in the levels of 8-OHdG which \ris the indicator of oxidative DNA damage. | en_US |
| dc.identifier.doi | 10.18038/aubtdc.802794 | |
| dc.identifier.endpage | 137 | en_US |
| dc.identifier.issn | 2667-4203 | |
| dc.identifier.issue | 2 | en_US |
| dc.identifier.scopusquality | N/A | |
| dc.identifier.startpage | 132 | en_US |
| dc.identifier.uri | https://doi.org/10.18038/aubtdc.802794 | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/18773 | |
| dc.identifier.volume | 10 | en_US |
| dc.identifier.wosquality | N/A | |
| dc.language.iso | en | en_US |
| dc.relation.ispartof | Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi C- yaşam Bilimleri ve Biyoteknoloji (Online) | en_US |
| dc.relation.publicationcategory | Makale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Biyoteknoloji Ve Uygulamalı Mikrobiyoloji | en_US |
| dc.subject | Biyokimya Ve Moleküler Biyoloji | en_US |
| dc.subject | Hücre Biyolojisi | en_US |
| dc.title | In Vitro Evaluation of the Effect of Glutathione on Caspase System \rand Oxidative Dna Damage In High Glucose Condition | en_US |
| dc.type | Article | en_US |