Coproduction of Biofuel and Pigments From Micractinium Sp. Using Uv-Induced Mutagenesis and Adding Abscisic Acid and Salicylic Acid for Biorefinery Concepts

Abstract

In this study, Micractinium sp. was randomly mutated by UV mutagenesis, and the contributions of Micractinium strains to the total biofuel and pigment production were investigated by adding abscisic and salicylic acid and examining the antioxidant activities of the strains to determine how they responded to these environmental and genetic alterations. As a result, compared with the control, the M-25 strain with 8 mg/L salicylic acid exhibited an increase of 54% in biodiesel production and 23% in biobutanol production, providing the maximum lipid percentage of 40%, whereas the M-7 strain and 0.2 mg/L abscisic acid showed an increase of 77% in biobutanol production while providing the highest carbohydrate percentage of 23%. The maximum total biofuel production was 139% for M-25 at 8 mg/L salicylic acid, and the highest amount of carotenoids (10.8 mg/g) was found to be approximately twice as high in the M-25 strain at 0.2 mg/L abscisic acid compared with the control.