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Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos

dc.authorscopusid 56342599500
dc.authorscopusid 57193389674
dc.contributor.author Köktürk, M.
dc.contributor.author Altındağ, F.
dc.date.accessioned 2025-05-10T16:55:19Z
dc.date.available 2025-05-10T16:55:19Z
dc.date.issued 2024
dc.department T.C. Van Yüzüncü Yıl Üniversitesi en_US
dc.department-temp Köktürk M., Department of Organic Agriculture Management, Faculty of Applied Sciences, Igdir University, Igdir, Turkey, Research Laboratory Application and Research Center (ALUM), Igdir University, Igdir, Turkey; Altındağ F., Department of Histology and Embryology, Faculty of Medicine, Van Yüzüncü Yıl University, Van, Turkey en_US
dc.description.abstract Zebrafish embryos are an important organism used as an in vivo model in a wide variety of disciplines from the past to the present. Immunohistochemistry analyses are an important method used to determine the localization of specific antigens in tissue sections with labeled antibodies depending on antigen-antibody interactions in zebrafish embryos. Immunofluorescence assays are an immunohistochemistry method that uses fluorophores to determine diverse cellular antigens. Zebrafish embryos and larvae, with their small size, are the most ideal model organisms for whole-mount immunohistochemical and immunofluorescent methods today. The small size of these organisms allows simultaneous evaluation of different tissues and organs, and results are obtained in a shorter time. In this section, whole-mount immunohistochemical and immunofluorescent analysis methods in zebrafish embryos, and larvae are summarized in detail, taking into account different studies and recent advances. © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024. en_US
dc.identifier.doi 10.1007/978-1-0716-3625-1_24
dc.identifier.endpage 407 en_US
dc.identifier.issn 1064-3745
dc.identifier.pmid 38285355
dc.identifier.scopus 2-s2.0-85184345537
dc.identifier.scopusquality Q4
dc.identifier.startpage 403 en_US
dc.identifier.uri https://doi.org/10.1007/978-1-0716-3625-1_24
dc.identifier.uri https://hdl.handle.net/20.500.14720/3452
dc.identifier.volume 2753 en_US
dc.identifier.wosquality N/A
dc.language.iso en en_US
dc.publisher Humana Press Inc. en_US
dc.relation.ispartof Methods in Molecular Biology en_US
dc.relation.publicationcategory Kitap Bölümü - Uluslararası en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.subject Immunofluorescence en_US
dc.subject Whole-Mount Immunohistochemistry en_US
dc.subject Zebrafish Embryos en_US
dc.title Whole-Mount Immunohistochemical and Immunofluorescence Assays in Zebrafish Embryos en_US
dc.type Book Part en_US

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