Investigation of Rotenone Effects on Ovarian Tissue Using Stereological Methods

Abstract

This study aims to stereologically investigate the effects of rotenone on rat ovarian tissue. Within the scope of the study, eighteen female rats were taken and divided into 3 groups as control group, to which physiological saline was administered, vehicle group to which sunflower oil was administered, and rotenone group to which rotenone dissolved in sunflower oil was administered at a dose of 2 mg/kg subcutaneously (s.c.). At the end of the experiment the right ovarian tissue was excised and removed, and kept in 10% formaldehyde for 72 hours. Then routine histological follow-up process were applied, and the tissues were embedded in paraffin. The sections of 5µm thickness were taken and stained with Haematoxylin-Eosin (H&E) and Masson's trichrome viewed under a light microscope and photographed. When the histological structure of the ovarian tissue in the rotenone group was examined, it was observed that there was a remarkably decrease in the number of follicles. When the total ovarian tissue volumes of the groups were compared stereologically, it was determined that the total ovarian tissue volume decreased in the rotenone group and this decrease was statistically significant (p<0.05). As a result, it was determined in our study that rotenone has harmful effects on the ovary, which is a part of the female reproductive system. © 2024, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.