Production of Trans-Resveratrol in 'cabernet Sauvignon' (Vitis Vinifera L.) Callus Culture in Response To Ultraviolet-C Irradiation

Abstract

In this study, the effects of ultraviolet (UV) irradiation time, incubation time and callus age were investigated for the induction of trans-resveratrol production in callus cultures of Vitis vinifera L. 'Cabernet Sauvignon'. Callus tissues were exposed to 254 nm UV light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. High Pressure Liquid Chromatography (HPLC) was used for the determination of trans-resveratrol production and concentrations were recorded at 0, 24, 48 and 72 hours after beginning of incubation. Separation by HPLC was achieved using a C18 column and a gradient elution with acetonitrile and water (from 10 to 85% acetonitrile). The peak of trans-resveratrol was detected at 330 nm and identified from the retention time (12.5 min) trans-resveratrol standard. Determination coefficient for linearity (R-2), Limit of Detections (LOD), Limit of Quantification (LOQ) and relative standard deviation (RSD) values of the method were found as 0.9994, 0.12, 0.35 and 1.9, respectively. The highest trans-resveratrol concentration (62.66 mu g.g(-1) callus fresh weight) was determined in 48 hours of 12 days-old callus cultures irradiated for 10 minutes. Both 10 min and 15 min UV irradiation periods were found to be effective for the induction of trans-resveratrol production and thus callus cultures could be convenient for trans-resveratrol production.