Changing of Flavan Contents at Some Organs of Walnut Seedlings (Juglans Regia L) Exposed To the Controlled Grafting Conditions

Abstract

In this study, changing of the flavans, which are one of the phenolic compounds, in the shoot tips, the roots and the bases of lateral branches was followed. The flavan changes after 1, 15, 30 and 45 days in the organs of two years old seedlings of walnut (Juglans regia L.) under controlled grafting conditions with 26+/-2 degrees C temperature and 60-70 % humidity were determined by TLC (Thin Layer Chromatography). The observations in the first 30 days were evaluated in relation to the anatomical structure of the graft union, and the changes of 50 days were considered connected with changing of the phenolic compounds in case of exposing to the controlled conditions. Consequently, the flavans decreased 35.95 % in the shoot tips, 32.68 % in the bases of lateral branches and 19.38 % in the roots in first 30 days. But, they increased 48.86 % in the shoot tips and 34.29 % at the roots while reducing 68.81 % in the bases of lateral branches from 30th day to 50th day. In addition, from the 1st day to 50th day they showed a decrease of 4.66 % in the shoot tips and of 79.00 % in the base of lateral branches. but an increase of 8.27 % in the root.