Radical Scavenging and Antioxidant Activity of Tannic Acid

Abstract

Tannic acid, a naturally occurring plant polyphenol, is composed of a central glucose molecule derivatized at its hydroxyl groups with one or more galloyl residues. In the present paper, we examines the in vitro radical scavenging and antioxidant capacity of tannic acid by using different in vitro analytical methodologies such as 1,1-diphenyl-2-picryl-hydrazyl free radical (DPPH center dot) scavenging, 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, total antioxidant activity determination by ferric thiocyanate, total reducing ability determination using by Fe3+-Fe2+ transformation method, superoxide anion radical scavenging by riboflavin-methionine-illuminate system, hydrogen peroxide scavenging and ferrous ions (Fe2+) chelating activities. Also, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), alpha-tocopherol and trolox, a water-soluble analogue of tocopherol, were used as the reference antioxidant radical scavenger compounds. Tannic acid inhibited 97.7% lipid peroxidation of linoleic acid emulsion at 15 mu g/mL concentration. On the other hand, the above mentioned standard antioxidants indicated an inhibition of 92.2%, 99.6%, 84.6% and 95.6% on peroxidation of linoleic acid emulsion at 45 mu g/mL concentration, respectively. In addition, tannic acid had an effective DPPH? scavenging, ABTS(center dot+) radical scavenging, superoxide anion radical scavenging, hydrogen peroxide scavenging, Fe3+ reducing power and metal chelating on ferrous ions activities. Also, those various antioxidant activities were compared to BHA, BHT, alpha-tocopherol and trolox as references antioxidant compounds. The present study shows that tannic acid is the effective natural antioxidant component that can be used as food preservative agents or nutraceuticals. (C) 2009 King Saud University. All rights reserved.