The Effects of Uv Irradiation and Incubation Time on in Vitro Phenolic Compound Production in 'karaerik Grape Cultivar

Abstract

In this study, the effect of Ultraviolet (UV) irradiation on induction of individual and total phenolics production on callus cultures of 'Karaerik' grape cultivar was investigated. Callus tissues were obtained from the leaves of the cuttings grown in in vitro plants. As a culture medium, Gamborg B-5 was utilized with 0.1 mg L-1 NAA (Naphthaleneacetic acid) and 0.2 mg L-1 Kin (Kinetin). Callus tissues were sub-cultured twice with 21 days intervals. After the second subculture, 12-day-old callus tissues were exposed to 254 nm UV-C light at 10 cm distance from the source for 10 and 15 min by opening covers of the petri dishes in sterile cabin. After the treatment, callus tissues were incubated under dark conditions. Phenolic compounds were measured at 24th, 48th and 72nd hours. Individual phenolic compounds were analyzed by HPLC (High Pressure Liquid Chromatography) and total phenolic compounds were measured by spectrophotometer. As a result of the study, it was found that UV irradiation was effective for induction the production of phenolic compounds in the callus tissues of 'Karaerik' grape cultivar and this effect was closely related to the application time.