Protective Effects of Naringin on Liver Enzymes (Ast, Alt, Alp) and Histopathology in Cyclophosphamide-Induced Rats

Abstract

In this study, it is aimed to investigate the protective effects of Naringin on liver enzymes (Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT), Alkaline Phosphatase (ALP) and histopathology in CYP-induced rats. In our study, forty male adult Sprague Dawley rat weighing approximately 200-250 g were used and 5 gropus formed. Analyzes of liver enzymes were done in auto analyzer and spectrophotometer. The liver tissue samples stained with hematoxylin eosin were examined by light microscopy. CYP was found to cause hapatotoxicity and showed increased liver enzymes in rats. AST, ALT and ALP values were 58±11, 32±8, 38±6 in the control group; 237±42, 168±44, 74±11 in the CYP group; 223±33, 158±42, 62±9 in the Naringin 50+CYP group; 117±25, 107±24, 48±8 in Naringin 100+CYP group; 54±9, 31±7, 36±6 in the group of Naringin 100 were found respectively. According to the values obtained from the control group, there is an increase of P<0.001 in all of the AST, ALT and ALP values in CYP-treated groups. Degenerative and necrotic tissue damage caused by CYP in the liver tissue was found to be reduced as dose-dependent with effect of Naringin. In conclusion, different doses of Naringin were determined to be protective effective for CYP-induced hepatotoxicity. © 2018 Ataturk Universitesi.All Rights Reserved.