Callus Formation From Isolated Microspore Culture in Radish (Raphanus Sativus L.)
dc.authorscopusid | 57188850112 | |
dc.contributor.author | Tuncer, B. | |
dc.date.accessioned | 2025-05-10T17:49:12Z | |
dc.date.available | 2025-05-10T17:49:12Z | |
dc.date.issued | 2017 | |
dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
dc.department-temp | [Tuncer, B.] Yuzuncu Yil Univ, Dept Hort, Fac Agr, Van, Turkey | en_US |
dc.description.abstract | In this study, the effects of applications of colchicine on microspore cultures were evaluated in 4 radish (Raphanussativus L.) varieties. For this purpose, 5 different concentrations of colchicine (0, 10, 25, 50, and 75 mg L-1) were applied under dark conditions at 4 degrees C to microspores that had been isolated in Gamborg's medium containing 13% (w/v) sucrose (B5-13). After the application of colchicine the microspores were kept for 1 day at 32.5 degrees C in1/2 x Nitsch and Nitschliquid media supplemented with13% (w/v) sucrose (1/2 NLN-13) at a density of 40.000 microspore mL(-1)and transferred for culturing under dark conditions at 25 degrees C. Colonies of callus were observed in Petri dishes 4-5 weeks after the microspore isolation. In this study, the 50 mg L-1 dose of colchicine was found to be more effective in terms of callus formation. The highest callus formation was achieved from the large round radish (cv. Burkir) (6.63 calli/Petri dish) and the standard small rooted radish (cv. Cherry Belle)(5.73 calli/Petri dish) varieties. | en_US |
dc.description.sponsorship | Yuzuncu Yil University Scientific Research Project Council (YYU BAP) [2015-ZF-B052] | en_US |
dc.description.sponsorship | This study was supported by Yuzuncu Yil University Scientific Research Project Council (YYU BAP, Project No: 2015-ZF-B052). | en_US |
dc.description.woscitationindex | Science Citation Index Expanded | |
dc.identifier.endpage | 282 | en_US |
dc.identifier.issn | 1018-7081 | |
dc.identifier.issn | 2309-8694 | |
dc.identifier.issue | 1 | en_US |
dc.identifier.scopus | 2-s2.0-85014665437 | |
dc.identifier.scopusquality | Q3 | |
dc.identifier.startpage | 277 | en_US |
dc.identifier.uri | https://hdl.handle.net/20.500.14720/17388 | |
dc.identifier.volume | 27 | en_US |
dc.identifier.wos | WOS:000398123000034 | |
dc.identifier.wosquality | Q3 | |
dc.institutionauthor | Tuncer, B. | |
dc.language.iso | en | en_US |
dc.publisher | Pakistan Agricultural Scientists Forum | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.title | Callus Formation From Isolated Microspore Culture in Radish (Raphanus Sativus L.) | en_US |
dc.type | Article | en_US |