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Callus Formation From Isolated Microspore Culture in Radish (Raphanus Sativus L.)

dc.authorscopusid 57188850112
dc.contributor.author Tuncer, B.
dc.date.accessioned 2025-05-10T17:49:12Z
dc.date.available 2025-05-10T17:49:12Z
dc.date.issued 2017
dc.department T.C. Van Yüzüncü Yıl Üniversitesi en_US
dc.department-temp [Tuncer, B.] Yuzuncu Yil Univ, Dept Hort, Fac Agr, Van, Turkey en_US
dc.description.abstract In this study, the effects of applications of colchicine on microspore cultures were evaluated in 4 radish (Raphanussativus L.) varieties. For this purpose, 5 different concentrations of colchicine (0, 10, 25, 50, and 75 mg L-1) were applied under dark conditions at 4 degrees C to microspores that had been isolated in Gamborg's medium containing 13% (w/v) sucrose (B5-13). After the application of colchicine the microspores were kept for 1 day at 32.5 degrees C in1/2 x Nitsch and Nitschliquid media supplemented with13% (w/v) sucrose (1/2 NLN-13) at a density of 40.000 microspore mL(-1)and transferred for culturing under dark conditions at 25 degrees C. Colonies of callus were observed in Petri dishes 4-5 weeks after the microspore isolation. In this study, the 50 mg L-1 dose of colchicine was found to be more effective in terms of callus formation. The highest callus formation was achieved from the large round radish (cv. Burkir) (6.63 calli/Petri dish) and the standard small rooted radish (cv. Cherry Belle)(5.73 calli/Petri dish) varieties. en_US
dc.description.sponsorship Yuzuncu Yil University Scientific Research Project Council (YYU BAP) [2015-ZF-B052] en_US
dc.description.sponsorship This study was supported by Yuzuncu Yil University Scientific Research Project Council (YYU BAP, Project No: 2015-ZF-B052). en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.endpage 282 en_US
dc.identifier.issn 1018-7081
dc.identifier.issn 2309-8694
dc.identifier.issue 1 en_US
dc.identifier.scopus 2-s2.0-85014665437
dc.identifier.scopusquality Q3
dc.identifier.startpage 277 en_US
dc.identifier.uri https://hdl.handle.net/20.500.14720/17388
dc.identifier.volume 27 en_US
dc.identifier.wos WOS:000398123000034
dc.identifier.wosquality Q3
dc.institutionauthor Tuncer, B.
dc.language.iso en en_US
dc.publisher Pakistan Agricultural Scientists Forum en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.title Callus Formation From Isolated Microspore Culture in Radish (Raphanus Sativus L.) en_US
dc.type Article en_US

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