PubMed İndeksli Yayınlar Koleksiyonu

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Antioxidant, Antimicrobial, Cytotoxic and Protective Effects of Truffles
(Academic Press inc Elsevier Science, 2022) Fidan, Mehmet; Ali, Muhammad Muddassir; Erez, Mehmet Emre; Cigerci, Ibrahim Hakki; Ozdemir, Sadin; Sen, Fatih
Fungi can be used as a potent chemotherapeutic agent to treat various cancers. In current study acetone and methanol extracts of Terfezia claveryi, Terfezia boudieri, Terfezia olbiensis, Picoa lefebvrei, Picoa juniperi were used to assess total phenolic contents, antioxidant activity, ion-chelating impact, antimicrobial activity, the cytotoxic and protective effects. Both methanol and acetone extracts of T. boudieri had the highest FRAP and DPPH scavenging abilities. Dose-dependent increased ion-chelating impact of all tested truffles species was found. Extracts of T. boudieri, T. claveryi, and T. albiensis exhibited higher antimicrobial activities. T. claveryi and T. boudieri showed the highest protective effects against H2O2-induced genotoxicity (P < 0.05), in S. cerevisiae BY4741. The least protective effect was showed by the acetone extracts of T. olbiensis (144 +/- 8); methanol ex-tracts of P. lefebvrei (140 +/- 8) and P. juniperi (140 +/- 10). MCF 7 cells showed more sensitivity against to methanol extracts of T. boudieri at 10-100 mu g/mL concentrations. HepG2 cells showed more sensitivity against the methanolic extracts of T. boudieri at both doses. Overall, P. lefebvrei and P. juniperi extracts had the least cytotoxic effects. The species of Terfezia exhibit significant protective effects against DNA damage and also have the potential of cytotoxicity effects.
Assessment of Stigmatization and Self-Esteem in Patients With Epilepsy
(Academic Press inc Elsevier Science, 2024) Atan, Gulden; Oruc, Fatma Gunduz; Atan, Kemal
Aim: This study was conducted to examine the relationship between stigmatization and self-esteem of patients with epilepsy. Methods: This cross-sectional study was conducted with 216 patients at a university hospital in Van, a province in eastern Turkey. The researcher prepared the data collection tools, including a personal information form, Jacoby Stigma Scale and the Rosenberg self-esteem scale, in line with the literature. The Jacoby stigma scale was applied to assess stigma. Data analysis was performed using t-test, one-way analysis of variance (ANOVA), and Tukey, LSD coefficient. Results: The mean total stigmatization score of patients with epilepsy was 21.11 +/- 10.00, while the mean total self-esteem score was 20.26 +/- 5.16. The study found a significant negative correlation between stigmatization and self-esteem scores (r = -0.411; p < 0.05). Conclusion: The research study revealed that patient with epilepsy have a high perception of stigma and low selfesteem levels.
Carbon-Nanotube Rhodium Nanoparticles as Highly-Active Catalyst for Hydrolytic Dehydrogenation of Dimethylamineborane at Room Temperature
(Academic Press inc Elsevier Science, 2018) Gunbatar, Serdar; Aygun, Aysenur; Karatas, Yasar; Gulcan, Mehmet; Sen, Fatih
In this study, we present a carbon nanotube-based Rh nanomaterial as a highly active catalyst for the hydrolytic dehydrogenation of dimethylamine - borane (DMAB) at room temperature. The prepared multi-walled carbon nanotube based Rh nanoparticles, called Rh NPs@ MWCNT, was readily prepared, stabilized and effectively used for the hydrolytic dehydrogenation of DMAB under ambient conditions. Monodisperse Rh NPs@ MWCNT nanocatalyst was characterized by using advanced analytical methods such as X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HR-TEM) etc. These analytical methods revealed that Rh nanoparticles on the surface of MWCNT were well dispersed and the average particle size was found to be 1.44 +/- 0.17 nm. The catalytic experiments revealed that the new Rh NPs@MWCNT nanocatalyst has a high catalytic effect to obtain hydrogen in 3.0 equation from DMAB, and the record catalytic TOF value for the catalytic reaction catalyzed by Rh NPs@MWCNT nanocatalyst was found to be 3010.47 h(-1) at room temperature. The current study presents the detailed kinetic studies of the hydrolytic dehydrogenation of DMAB catalyzed by Rh NPs@MWCNT, the results of catalytic experiments were performed at different temperatures, substrate and catalyst concentrations, the Rh NPs@MWCNT nanocatalyst was effectively used in the completion of the hydrolytic dehydrogenation of DMAB, and activation energy, enthalpy and entropy parameters. The experimental results showed that monodisperse Rh NPs@MWCNT nanocatalyst have record catalytic activity with TOF value of 3010.47 h(-1), and Rh(0) nanoparticles were well dispersed on the multi-walled carbon nanotubes. (C) 2018 Elsevier Inc. All rights reserved.
Characterization of Peripheral Blood T Follicular Helper (Tfh) Cells in Patients With Type 1 Gaucher Disease and Carriers
(Academic Press inc Elsevier Science, 2023) Uzen, Ramazan; Bayram, Fahri; Dursun, Huseyin; Kardas, Fatih; Cakir, Mustafa; Cucer, Nurhan; Donmez-Altuntas, Hamiyet
Background: Gaucher disease (GD) is the most common autosomal recessive lipid storage disease. In this study, the changes in TFH cells and IL-4 and IL-21 cytokines in blood samples of GD patients, carriers and healthy volunteers were investigated.Methods: Two pretreatment type 1 GD patients, 20 currently treated type 1 GD patients, 6 carriers, and 27 healthy volunteers were enrolled in the study. TFH cell (CD45RA- CD4+CXCR5+) number, phenotype (PD1, ICOS expression), and cytokine production (IL-21, IL-4) were assessed via flow cytometric assays.Results: No significant differences were found between the groups with respect to the number, frequency and PD1 or ICOS expression of TFH cells between healthy controls, patients and carriers. However, IL-4+ TFH cells were significantly reduced both in percent and number in the treated GD patients compared with healthy controls (p < 0.05). Interestingly, the IL-21+ TFH cell number was increased in treated GD patients. When TFH cells were examined based on CXCR3 expression, the frequency of the PD1+Th17-Th2-like fraction (CXCR3-) was found to be significantly increased in treated GD patients. Conclusion: To our knowledge, this is the first study to assess TFH cells in GD patients, and to show that the production of IL-4 and IL-21 by TFH cells and their subsets may be altered in type 1 GD patients.
Characterization of Rheum Ribes With Zno Nanoparticle and Its Antidiabetic, Antibacterial, Dna Damage Prevention and Lipid Peroxidation Prevention Activity of in Vitro
(Academic Press inc Elsevier Science, 2022) Meydan, Ismet; Burhan, Hakan; Gur, Tugba; Seckin, Hamdullah; Tanhaei, Bahareh; Sen, Fatih
This study aims to investigate the antidiabetic, antimicrobial, DNA damage, and lipid peroxidation prevention activity of ZnO NPs/Rr formed as a result of the interaction of Rheum ribes (R.ribes) plant with ZnO. The ZnO NPs/Rr obtained as a result of the reaction were confirmed using high-reliability characterization methods. According to the data obtained as a result of the study, it is seen that the activity of ZnO NPs/Rr to prevent lipid peroxidation is quite strong. Lipid peroxidation inhibition activity of ZnO NPs/Rr at the highest concentration of 250 mu g/ml was calculated as % 89.1028. It was observed that ZnO NPs/Rr prevented DNA damage by % 92.1240 at the highest concentration of 100 mu g/ml. It was determined that the antidiabetic effect of ZnO NPs/Rr formed by ZnO of R. ribes plant, which is used as a medicinal plant as an antidiabetic, was significant. It appears to have a strong antidiabetic property compared to the positive control acarbose. In our current study, it was observed that ZnO NPs/Rr formed zones ranging from 8 +/- 3.0 to 21 +/- 4.5 against Gram-positive and Gram-negative microorganisms. It has been determined that ZnO nanoparticles have an antibacterial effect.
Comparison of Two Methods (Microscopy and Enzyme-Linked Immunosorbent Assay) for the Diagnosis of Amebiasis
(Academic Press inc Elsevier Science, 2005) Tanyuksel, M; Yilmaz, H; Ulukanligil, M; Araz, E; Cicek, M; Koru, O; Petri, WA
Diagnosis of amebiasis is usually performed on a clinical basis alone in most endemic countries having limited economic resources. This epidemiological study was conducted using modern diagnostic tests for amebiasis in the southeastern region of Turkey, an endemic area for amebiasis. The population of this study included patients with symptomatic diarrhea/dysentery attending both Yuzuncu Yil University, Van and Harran University, Sanliurfa, Turkey. A total of 380 stool specimens were collected and examined for Entamoeba by light microscopy (fresh, lugol, and trichrome staining) and stool antigen detection based- enzyme-linked immunosorbent assay (EIA) test (TechLab Entamoeba histolytica 11). 24% (91/380) of stool specimens were positive for E. histolytical Entamoeba dispar trophozoites/cysts microscopically using trichrome staining. 13% (51/380) of the stool specimens were found to be positive for E. histolytica by the EIA test, including 15% (14/91) of microscopy (+) stool specimens and 13% (37/289) of microscopy (-) stool specimens. Enteric parasites were common in these populations with 66% (251/380) of the study population harboring more than one parasite. In addition to the 13% (51/380) of patients determined to have E. histolytica by EIA, eighty-six patients (22.6%) had Blastocystis hominis, 54 (14.2%) Entamoeba coli, 44 (11.5%) Giardia lamblia, 16 (4.2%) Chilomastix mesnili, 15 (3.9%) Iodamoeba butschlii, 12 (3.1%) Hymenolepis nana, 9 (2.3%) Endolimax nana, 9 (2.3%) Dientamoeba fragilis, and 8 (2.1%) had Ascaris lumbricoides. We concluded that E histolytica infection was found in 13% of the patients presenting with diarrhea in Van and Sanliurfa Turkey. (c) 2005 Elsevier Inc. All rights reserved.
Composition Dependent Activity of Pdagni Alloy Catalysts for Formic Acid Electrooxidation
(Academic Press inc Elsevier Science, 2018) Ulas, Berdan; Caglar, Aykut; Sahin, Ozlem; Kivrak, Hilal
In the present study, the carbon supported Pd, PdAg and PdAgNi (Pd/C, PdAg/C and PdAgNi/C) electrocatalysts are prepared via NaBH4 reduction method at varying molar atomic ratio for formic acid electrooxidation. These as-prepared electrocatalysts are characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), inductively coupled plasma mass spectrometry (ICP-MS), N-2 adsorption desorption, and X-ray electron spectroscopy (XPS), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), chronoamperometry (CA), and lineer sweep voltammetry (LSV). While Pd-50,Ag-50/C exhibits the highest catalytic activity among the bimetallic electrocatalyst, it is observed that Pd70Ag20Ni10/C electrocatalysts have the best performance among the all electrocatalysts. Its maximum current density is about 1.92 times higher than that of Pd/C (0.675 mA cm(-2)). Also, electrochemical impedance spectroscopy (EIS), chronoamperometry (CA) and lineer sweep voltammetry (LSV) results are in a good agreement with CV results in terms of stability and electrocatalytic activity of Pd50Ag50/C and Pd70Ag20Ni10/C. The Pd70Ag20Ni10/C catalyst is believed to be a promising anode catalyst for the direct formic acid fuel cell. (C) 2018 Elsevier Inc. All rights reserved.
Determination of Complementary and Alternative Medicine Use Frequency and Related Factors in Children With Epilepsy: a Descriptive Cross-Sectional Study From Eastern Turkey
(Academic Press inc Elsevier Science, 2024) Can, Veysel; Bulduk, Mehmet; Aysin, Nesrullah; Can, Emine Kurt; Aydin, Nihal
Purpose: The aim of this study is to determine the complementary and alternative medicine (CAM) methods used in epilepsy patients admitted to a paediatric neurology clinic in Turkey and to examine the factors affecting these methods. Methods: This study was conducted between October 2022 and March 2023 by using a questionnaire form prepared in line with the literature to the parents (n = 172) of children who were under the age of 18 and who had been diagnosed with epilepsy for at least 1 year and who were followed up in the paediatric neurology outpatient clinic of a secondary care hospital in Van province of Turkey. The principles of scientific ethics and confidentiality of data were complied with in all stages of the study. Results: The study included 172 parents of 172 children with a mean age of 2.95 +/- 0.93 years. In the study, it was found that 37.2 % of the parents had previously used CAM themselves, almost all of the parents (98.8 %) had not discussed the use of CAM with their physician or nurse, half of the parents considered CAM to be dangerous, and 28.2 % wanted to receive counselling to learn more about CAM. It was also found that 28.5 % of the parents used CAM in the treatment of their children. The most commonly preferred CAM among parents in the treatment of epilepsy was religious healing (79.6 %) which is among mind/body practices. It was found that the most common technique used by parents was regular praying (71.4 %). Religious beliefs were effective in the use of CAM in our patient population, while confidence in medical treatment was effective in not using CAM. More than half of the parents who used CAM in their child's treatment reported benefits and reduced seizure frequency. The factors significantly associated with CAM use in children with epilepsy were parental education level, parental CAM use and disease duration. In univariate and multivariate analyses, it was found that a degree below high school increased the likelihood of CAM use by 0.017 and 0.024 times, respectively, compared to being illiterate. Conclusions: Pediatric nurses' routine examination of CAM methods used in paediatric epilepsy patients and the factors affecting these methods is very important in terms of preventing negative effects on child health, effective management of the disease process, ensuring safe use and strengthening communication to improve the quality of care.
Doxorubicin-Induced Senescence Promotes Resistance To Cell Death by Modulating Genes Associated With Apoptotic and Necrotic Pathways in Prostate Cancer Du145 Cd133+/Cd44+cells
(Academic Press inc Elsevier Science, 2023) Tatar, Cansu; Avci, Cigir Biray; Acikgoz, Eda; Oktem, Gulperi
Cancer stem cells (CSCs) are the most important cause of cancer treatment failure. Traditional cancer treatments, such as chemotherapy and radiotherapy, damage healthy cells alongside malignant cells, leading to severe adverse effects. Therefore, inducing cellular senescence without triggering apoptosis, which further damages healthy cells, may be an alternative strategy. However, there is insufficient knowledge regarding senescence induction in CSCs that show resistance to treatment and stemness properties. The present study aims to elucidate the effects of senescence induction on proliferation, cell cycle, and apoptosis in prostate CSCs and non-CSCs. Prostate CSCs were isolated from DU145 cancer cells using the FACS method. Subsequently, senescence induction was performed in RWPE-1, DU145, prostate CSCs, and non-CSCs by using different concentrations of Doxorubicin (DOX). Cellular senescence was detected using the senescence markers SA-beta-gal, Ki67, and senescence-associated heterochromatin foci (SAHF). The effects of senescence on cell cycle and apoptosis were evaluated using the Muse Cell Analyzer, and genes in signaling pathways associated with the apoptotic/necrotic pathway were analyzed by real-time PCR. Prostate CSCs were isolated with 95.6 +/- 1.4% purity according to CD133+/CD44+ characteristics, and spheroid formation belonging to stem cells was observed. After DOXinduced senescence, we observed morphological changes, SA-beta-gal positivity, SAHF, and the lack of Ki67 in senescent cells. Furthermore; we detected G2/M cell cycle arrest and downregulation of various apoptosis-related genes in senescent prostate CSCs. Our results showed that DOX is a potent inducer of senescence for prostate CSCs, inhibits proliferation by arresting the cell cycle, and senescent prostate CSCs develop resistance to apoptosis.
The Effect of Ginkgo Extract Egb761 in Cisplatin-Induced Peripheral Neuropathy in Mice
(Academic Press inc Elsevier Science, 2004) Öztürk, G; Anlar, Ö; Erdogan, E; Kösem, M; Özbek, H; Türker, A
Neuroprotective effect of Ginkgo biloba extract EGb761 in cisplatin (cis-diamminedi-chloroplatinum, or CDDP)-induced peripheral neuropathy was investigated. Swiss albino mice were treated with CDDP, 2 mg/kg ip twice a week for nine times. One group of the animals also received EGb761 in the drinking water at an estimated dosage of 100 mg/kg per day. Two other groups received vehicle (control) or EGb761 only. Development of neuropathy was evaluated with changes in sensory nerve conduction velocity (NCV). Following the treatments, dorsal root ganglia (DRGs) were microscopically examined and some were cultured for 3 days. EGb761 proved effective in preventing the reduction in NCV (P < 0.0001) caused by CDDP. CDDP caused a decrease in the number of migrating cells (P < 0.01) and in the length of outgrowing axons (P < 0.01) while EGb761 treatment prevented the latter. CDDP led to smaller nuclear and somatic sizes in neurons (P < 0.01), while with EGb761 co-administration, both were close to control values. Animals having EGb761 only had similar results with controls. In conclusion, EGb761 was found to be effective in preventing some functional and morphological deteriorations in CDDP-induced peripheral neuropathy. (C) 2004 Elsevier Inc. All rights reserved.
Effect of Surgical Staging on 539 Patients With Borderline Ovarian Tumors: a Turkish Gynecologic Oncology Group Study
(Academic Press inc Elsevier Science, 2013) Guvenal, Tevfik; Dursun, Polat; Hasdemir, Pinar S.; Hanhan, Merih; Guven, Suleyman; Yetimalar, Hakan; Ayhan, Ali
Objective. The objectives of this study were to examine demographic and clinicopathologic characteristics and to determine the effects of primary surgery, surgical staging and the extensiveness of staging. Methods. In a retrospective Turkish multicenter study, 539 patients, from 14 institutions, with borderline ovarian tumors were investigated. Some of the demographic, clinical and surgical characteristics of the cases were evaluated. The effects of type of surgery, surgical staging; complete or incomplete staging on survival rates were calculated by using Kaplan-Meier method. Results. The median age at diagnosis was 40 years (range 15-84) and 71.1% of patients were premenopausal. The most common histologic types were serous and mucinous. Majority of the staged cases were in Stage IA (735%). 242 patients underwent conservative surgery. Recurrence rates were significantly higher in conservative surgery group (8.3% vs. 3%). Of all patients in this study, 294 (545%) have undergone surgical staging procedures. Of the patients who underwent surgical staging, 228 (77.6%) had comprehensive staging including lymphadenectomy. Appendectomy was performed on 204 (37.8%) of the patients. The median follow-up time was 36 months (range 1-120 months). Five-year survival rate was 100% and median survival time was 120 months. Surgical staging, lymph node sampling or dissection and appendectomy didn't cause any difference on survival. Conclusion. Comprehensive surgical staging, lymph node sampling or dissection and appendectomy are not beneficial in borderline ovarian tumors surgical management. (C) 2013 Elsevier Inc. All rights reserved.
Effective Eradication of Pinworms (Syphacia Obvelata and Aspiculuris Tetraptera) With Polygonum Cognatum Meissn
(Academic Press inc Elsevier Science, 2019) Dereli, Fatma Tugce Guragac; Ilhan, Mert; Kozan, Esma; Akkol, Esra Kupeli
Polygonum cognatum Meissn. (Polygonaceae) is a wild edible plant known locally as "solucanotu, madimak" in Turkey and it has been used for various purposes, such as diuretic and antidiabetic, as well to treat oxyuris and worms internally in humans. The present study was carried out to investigate the anthelmintic activity of various extracts were obtained from the aerial parts of P. cognatum in order to justify its traditional usage in Turkish folk medicine. n-Hexane, ethyl acetate and methanol extracts were prepared from the aerial parts of the plant, successively. The anthelmintic potentials of these extracts were evaluated on mice naturally infected with Syphacia obvelata and Aspiculuris tetraptera. Cellophane perianal tape test and fecal flotation test were used for the activity screening. The results were compared with control and reference groups. In addition to all these experiments, the total amount of phenolic compounds and tannin contents thought to be responsible for the activity were evaluated for all extracts. According to the results, it has been found that the methanolic extract reduced the number of S. obvelata (66.8%) and A. tetraptera (73.4%) eggs. Moreover, the methanolic extract include higher amount of total phenolic compound (48.75 +/- 0.82 mg of GA/g of extract) and tannin (30.04 +/- 0.22 mg TA/g of extract) than n-Hexane (17.21 +/- 0.75 mg of GA/g of extract; 6.82 +/- 0.41 mg TA/g of extract) and ethyl acetate (25.06 +/- 0.94 mg of GA/g of extract; 10.29 +/- 0.34 mg TA/g of extract); therefore the anthelmintic activity could be related to these constituents. The present study explored the anthelmintic potential of P. cognatum. Further studies should be conducted into the mechanism of these compounds' against helminths. This study can be an important step in the discovery of new anthelmintic agents.
Effects of Aminoguanidine and Antioxidant Erdosteine on Bleomycin-Induced Lung Fibrosis in Rats
(Academic Press inc Elsevier Science, 2004) Yildirim, Z; Turkoz, Y; Kotuk, M; Armutcu, F; Gurel, A; Iraz, M; Akyol, O
Reactive oxygen and nitrogen species have been implicated in the pathogenesis of bleomycin -induced lung fibrosis. The effects of aminoguanidine and erdosteine on the bleomycin-induced lung fibrosis were evaluated in rats. The animals were placed into five groups: Vehicle + vehicle, vehicle + bleomycin (2.5U/kg), bleomycin + aminoguanidine (200mg/kg), bleomycin + erdosteine (10mg/kg), and bleomycin + erdosteine + aminoguanidine. Bleomycin administration resulted in prominent lung fibrosis as measured by lung hydroxyproline content and lung histology, which is completely prevented by erdosteine and aminoguanidine. A strong staining for nitro tyrosine antibody in lung tissue and increased levels of lung NO were found in bleomycin group, that were significantly reduced by aminoguanidine and erdosteine. Aminoguanidine and erdosteine significantly prevented depletion of superoxide dismutase and glutathione peroxidase and elevated myeloperoxidase activities, malondialdehyde level in lung tissue produced by bleomycin. Data presented here indicate that aminoguanidine and erdosteine prevented bleomycin-induced lung fibrosis and that nitric oxide mediated tyrosine nitration of proteins plays a significant role in the pathogenesis of bleomycin-induced lung fibrosis. Also our data suggest that antifibrotic affect of antioxidants may be due to their inhibitory effect on nitric oxide generation in this model. (C) 2004 Elsevier Inc. All rights reserved.
The Effects of Exogenous Melatonin on Peripheral Nerve Regeneration and Collagen Formation in Rats
(Academic Press inc Elsevier Science, 2011) Atik, Bekir; Erkutlu, Ibrahim; Tercan, Mustafa; Buyukhatipoglu, Hakan; Bekerecioglu, Mehmet; Pence, Sadrettin
Background. Peripheral nerve damage that requires surgical repair does not result in complete recovery because of collagen scar formation, ischemia, free oxygen radical damage, and other factors. To date, the best treatment method has not yet been determined. In this study, we designed an experimental peripheral nerve injury model, and researched the possible effects of melatonin hormone, based on evidence of its strong antioxidant and cell-protective effects via mimicking the effects of calcium channel blockers. Materials and Methods. We randomized 24 healthy female albino rats into three groups: the pinealectomy group, melatonin group, and control group. In the pinealectomy group, craniotomy, pinealectomy, sciatic nerve transection, and coaptation were performed, and 0.9% NaCl was injected intraperitoneally. In the melatonin group, craniotomy (without pinealectomy), sciatic nerve dissection, and coaptation were performed, and melatonin was injected intraperitoneally, instead of NaCl. In the control group, craniotomy (without pinealectomy), sciatic nerve dissection and coaptation, and intraperitoneal NaCl injection were performed. In each group, nerve recovery was evaluated histologically, functionally, and electrophysiologically. Functional and electrophysiologic evaluations were conducted before surgery and at 4 and 12 wk. Results. At 4 wk, no significant difference was observed between the groups. However, at 12 wk, significant electrophysiologic and functional improvement was observed only in the melatonin group. Conclusions. Melatonin seems to have a beneficial effect on nerve recovery. However, this effect is not effective at physiologic doses. Future comparative studies with melatonin versus other nerve-regenerating agents are necessary to determine the clinical utility of melatonin hormone. (C) 2011 Elsevier Inc. All rights reserved.
Effects of Subacute Exposure of Dichlorvos at Sublethal Dosages on Erythrocyte and Tissue Antioxidant Defense Systems and Lipid Peroxidation in Rats
(Academic Press inc Elsevier Science, 2009) Celik, Ismail; Suzek, Huseyin
In this study, the effects of dichlorvos (DIC) at sublethal concentration on malondialdehyde (MDA) content and antioxidant defense system (ADS) such as reduced glutathione (GSH), glutathione reductase (GR), superoxide dismutase (SOD), and glutathione-S-transferase (GST) in various tissues of rats exposed to 0.0225 and 0.0450 millimole (mmol) DIC in drinking water were administered orally to rats ad libitum for 28 days continuously. MDA significantly increased in all the tissues except in the lungs of rats treated with both dosages of DIC. With regard to the ADS, GR significantly decreased in the erythrocytes whereas it did not change in the other tissues with two dosages of DIC. SOD activity significantly increased in the all tissues treated with both dosages of DIC. GST activity significantly increased in all the tissues except in the erythrocytes, spleen, and lungs treated with 0.0225mmol. Meanwhile, GSH depletion in the liver and heart of rats treated with 0.0225 mmol and all the tissues except in the brain and kidney of rats treated with 0.0450 mmol of DIC were found to be significant. The observations presented led us to conclude that the administrations of DIC promote MDA content and fluctuate in the ADS in rats during the exposure. (c) 2008 Elsevier Inc. All rights reserved.
An Environmental Approach for the Photodegradation of Toxic Pollutants From Wastewater Using Pt-Pd Nanoparticles: Antioxidant, Antibacterial and Lipid Peroxidation Inhibition Applications
(Academic Press inc Elsevier Science, 2022) Seckin, Hamdullah; Tiri, Rima Nour Elhouda; Meydan, Ismet; Aygun, Aysenur; Gunduz, Meliha Koldemir; Sen, Fatih
Background: Green synthesis is an effective and friendly method for the environment, especially in recent years has been used in many areas. It finds application opportunities in many fields such as physics, chemistry, electronics, food, and especially health and is the subject of intensive studies in this field. Objectives: The synthesized Pt-Pd NPs were aimed to be used as a bio-based photocatalyst under sunlight to prevent wastewater pollution. In addition, it is aimed to use Pt-Pd NPs as biological agents in different applications in the future. Methods: In this study, the platinum-palladium nanoparticles were synthesized by the extract of Hibiscus sabdariffa, the characterization of the nanoparticles was carried out by different methods (ultraviolet-visible spectroscopy (UV-vis), transmission electron microscopy (TEM), infrared transform spectroscopy atomic force microscopy (AFM), and ray diffraction (XRD) analysis). And we discussed several different parameters related to human health by obtaining platinum-palladium bimetallic nanoparticles (Pt-Pd NPs) with a green synthesis method. These parameters are antioxidant properties (total phenolic, flavonoid, and DPPH scavenging activity), antibacterial activity, and lipid peroxidation inhibition activity. Gallic acid was used as standard phenolic, and quercetin was used as standard flavonoid reagents. The newly synthesized Hibiscus sabdariffa mediated green synthesized Pt-Pd NPs were compared with gram-positive and gram-negative bacteria, the high antibacterial activity was shown by gram-positive bacteria. The photodegradation of Pt-Pd NPs was carried out against MB dye for 180 min. Results: TEM results show that the average size of Pt-Pd NPs is around 4.40 nm. The total amount of phenolic compounds contained in 0.2 mg/ml of Pt-Pd NPs was equivalent to 14.962 +/- 7.890 mu g/ml gallic acid and the total amount of flavonoid component was found to be equal to 28.9986 +/- 0.204 mu g/ml quercetin. Hibiscus sabdariffa mediated green synthesized Pt-Pd NPs was found to have very effective for lipid pemxidation inhibition activity in the FeCl2-H2O2 system. The maximum DPPH scavenging activity was determined as 97.35% at 200 mu g/mi. The photocatalytic activity of Pt-Pd NPs was analysed against Methylene blue (MB) and the maximum degradation percentage was observed to be 83.46% at 180 min. Conclusions: The biogenic Pt-Pd NPs showed a high effective photocatalytic and biological activity.
Ex Situ Synthesis and Characterization of a Polymer-Carbon Nanotube-Based Hybrid Nanocatalyst With One of the Highest Catalytic Activities and Stabilities for the Hydrolytic Dehydrogenation of Hydrazine-Borane at Room Temperature Conditions
(Academic Press inc Elsevier Science, 2019) Demirkan, Buse; Kuyuldar, Esra; Karatas, Yasar; Gulcan, Mehmet; Sen, Fatih
In this study, a facile ex situ synthesis of a polyaniline-multiwalled carbon nanotube-based Pt nanocatalyst (Pt@PANI-MWCNT) with an average particle size of 3.18 +/- 0.12 nm was performed successfully. The obtained Pt@PANI-MWCNT nanocatalysts were isolated from the solution medium by centrifugation and then were characterized by spectroscopy and microscopy methods. The characterization studies showed that the prepared Pt nanoparticles were formed on PANI-MWCNT surface, and H-2 evolution was obtained by the dehydrogenation of hydrazine-borane in water as a model reaction under room temperature conditions, with the help of the synthesized nanocatalyst. It was observed that the Pt@PANI-MWCNT nanocatalyst had a very high catalytic activity for the hydrolytic dehydrogenation of hydrazine-borane and generated 2.95 mol of H-2 for 1 mol of hydrazine-borane. The initial turn-over frequency (TOFinitial) value of the prepared nanocatalyst for the model reaction at room temperature conditions was found to be 168.5 min(-1). The calculations for the kinetics of the hydrolytic dehydrogenation reaction showed that the hydrazine-borane catalytic reaction kinetics are first order, with respect to the catalyst concentration; several activation parameters, such as entropy (Delta S-#, (app) = -72.11 +/- 3 J/mol K), enthalpy (Delta S-#, (app) = 43.5 +/- 2 kJ/mol) and activation energy (E-a,E- app = 45.5 +/- 2 kJ/mol), of the catalytic reaction with the Pt@PANI-MWCNT nanocatalyst were calculated using these kinetic data. (C) 2019 Elsevier Inc. All rights reserved.
Examination of the Level of Tolerance of Uncertainty and Psychological Wellness of Patients With Epilepsy
(Academic Press inc Elsevier Science, 2023) Atan, Gulden
Aim: This study was conducted to examine the level of intolerance of uncertainty and the psychological well-being of patients with epilepsy (PWE).Methods: This descriptive and cross-sectional study was conducted with 201 patients in a university hos-pital in Van, which is a province in eastern Turkey. Personal information form, intolerance of uncertainty scale, and the psychological well-being scale were prepared by the researcher in line with the literature and used to collect data. An independent sample t-test, one-way analysis of variance (ANOVA), post hoc (Tukey, LSD), correlation, and regression analyses were used to analyze the data.Results: In this study, the mean total score of PWE for the intolerance to uncertainty scale was found as 37.144 +/- 9.202. Among the sub-dimensions, the mean prospective anxiety score was found as 21.821 +/- 5. 416 and the mean inhibitory anxiety score was 15.323 +/- 4.695. The mean total score of the psychological well-being scale was found as 28.975 +/- 6.622. It was determined that there was a significant relationship between the total score of the intolerance of uncertainty scale and the total score of the psychological well-being scale of the PWE who participated in the study (F = 14.314; p = 0.000 < 0.05). It was observed that the total score of intolerance of uncertainty was decreased as the total level of psychological well-being was increased (ss =-0.186).Conclusion: It was found that PWE had higher levels of intolerance of uncertainty and lower levels of psy-chological well-being. It was also determined that the total score of psychological well-being was decreased as the total score of intolerance of uncertainty was increased.Recommendations: For preventing the negative consequences of intolerance of uncertainty in PWE, it is recommended to inform nurses to increase the level of psychological well-being as a result of raising awareness about their concerns by conducting studies to increase the level of intolerance to uncertainty.It is also recommended to carry out studies on intolerance of uncertainty and psychological well-being in advanced and larger sample groups. (c) 2023 Elsevier Inc. All rights reserved.
Expression of Laminin Receptor 1 in Gestational Trophoblastic Diseases and Normal Placenta and Its Relationship With the Development of Postmolar Tumors
(Academic Press inc Elsevier Science, 2009) Kurdoglu, Mertihan; Bayram, Irfan; Kolusari, Ali; Erten, Remzi; Adali, Ertan; Bulut, Gulay; Sahin, H. Guler
Objectives. To investigate the expression of laminin receptor 1 (LR1), a non-integrin-type laminin receptor, in gestational trophoblastic diseases and normal first-trimester placenta, since it may play a role in controlling trophoblast invasion in normal and molar pregnancies. Methods. Paraffin sections from 24 gestational age controlled normal first-trimester placentas, 47 partial moles, 56 complete moles, 3 invasive moles, 4 gestational choriocarcinomas, and 1 placental-site trophoblastic tumor were studied immunohistochemically for expression of LR1. Results. In complete and partial moles, decidual cells showed significantly stronger LR1 protein staining compared to the normal placenta (p<0.01). When compared to the partial moles, weak staining in less than 33% of decidual cells was also more prominent in the normal placenta (p<0.05). Complete and partial moles, invasive moles, choriocarcinomas, and placental-site tumors did not differ from each other with respect to staining intensity. Strong immunostaining for LR1 in decidual cells, cytotrophoblasts, syncytiotrophoblasts, and extracellular matrix cells of partial and complete moles was not significantly correlated with the development of persistent postmolar gestational trophoblastic tumors. Conclusions. LR1 may be important in the pathogenesis of gestational trophoblastic diseases. The increased expression of LR1 in decidual cells of partial and complete moles may not influence the development of persistent gestational trophoblastic tumor. Since they are seen rarely, multicentric studies should be planned to study LR1 expression in invasive moles and gestational trophoblastic neoplasms. (C) 2009 Elsevier Inc. All rights reserved.
Fabrication of Glucose Bioelectrochemical Sensor Based on Au@pd Core-Shell Supported by Carboxylated Graphene Oxide
(Academic Press inc Elsevier Science, 2023) Guler, Muhammet; Zengin, Adem; Alay, Murat
The study presents a novel electrochemical glucose biosensor based on glucose oxidase (GOx) immobilized on Au@Pd core-shell nanoparticles supported on carboxylated graphene oxide (cGO). The immobilization of GOx was achieved by cross-linking the chitosan biopolymer (CS) including Au@Pd/cGO and glutaraldehyde (GA) on a glassy carbon electrode. The analytical performance of GCE/Au@Pd/cGO-CS/GA/GOx was investigated using amperometry. The biosensor had fast response time (5.2 +/- 0.9 s), a satisfactory linear determination range between 2.0 x 10(-5) and 4.2 x 10(-3) M, and limit of detection of 10.4 mu M. The apparent Michaelis-Menten constant (K-app) was calculated as 3.04 mM. The fabricated biosensor also exhibited good repeatability, reproducibility, and storage stability. No interfering signals from dopamine, uric acid, ascorbic acid, paracetamol, folic acid, mannose, sucrose, and fructose were observed. The large electroactive surface area of carboxylated graphene oxide is a promising candidate for sensor preparation.

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