The Effects of Melatonin and Vitamin E on Antioxidant Parameters in Copper Nephrotoxicity

Abstract

Copper (Cu) compounds have been widely used in various fields especially industrial processes and agriculture. Although trace amounts of Cu are essential to sustain life, excessive Cu is toxic for living organisms. This study aims to investigate the effects of vitamin E and melatonin on antioxidant enzymes activities in kidney against acute Cu toxicity. In this study, 28 Wistar albino rats were divided four equal groups (n=7) as control, Cu, Cu+Vitamin E, and Cu+Melatonin groups. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) enzymes activities and protein carbonyl content were analyzed. Also, kidney function tests were evaluated. While Cu administration increased creatinine, blood urea nitrogen (BUN), uric acid, Na, K and Mg levels, vitamin E and melatonin was effective reduction on these levels. Carbonyl level significantly decreased in Cu+Vitamin E and Cu+melatonin groups compared to Cu group. Although SOD, GPx and CAT activities in tissue decreased by Cu exposure, vitamin E and melatonin treatment showed significant increase on their activities. Consequently, vitamin E and melatonin supplements may provide prevention Cu -based oxidation by strengthening the antioxidant defense system. In this respect, it may show a protective effect by preventing the development of nephrotoxicity. © Ataturk Universitesi. All rights reserved.