A Stereological Study: Volume Estimation in the Cerebellum of Norduz Sheep

Abstract

In this research, it was aimed to investigate the cerebellum volume values of Norduz sheep raised within the borders of Gurpinar district of Van province. For this purpose, 6 male and 6 female Norduz sheep heads were selected as materials. The brains were dissected and kept in 10% formaldehyde for a week. Cerebellums were separated from the brain hemispheriums. For better fixation, the cerebellums were kept in formaldehyde again for a week. Tissues obtained by sequential random sampling were included in tissue tracking. Then each tissue was embedded in paraffin. An average of 10-12 sections with a thickness of 5 mu m were obtained from the tissue cassettes obtained by a rotary microtome. Sections stained with hematoxylin and eosin were photographed with an x4 objective. From these photographs, total cerebellum, medulla and cortex volumes, as well as vermis volume, were calculated using the Shtereom I program using the Cavalieri Principle. The results were evaluated statistically with the Mann-Whitney U test. No statistical difference was detected in terms of male and female cerebellum volume values, Coefficient of Error (CE) and Noise calculations (p>0.05). However, only the left hemispherium cerebelli volume having a higher value in females was statistically significant (p<0.05).