Effects of Nitric Oxide-Vascular Endothelial Growth Factor Systems in Chick Embryo Cerebral Vasculogenesis and Angiogenesis

Abstract

Aim: Studies have depicted that nitric oxide and vascular endothelial growth factor systems have important biological effects in the development of vasculogenesis and angiogenesis. Material and Method: In this study; specific pathogen free white Leghorin type fertilised eggs were used. Result: Three main subgroups were designed regarding time points of 48; 72 and 80 hours of incubation. In 48 hours old embryos anjioblasts were seen; but no mature endothelial structure was present. In 72 hours old embryos endothelial maturation began. In the 80 hours there was a high level of endothelial maturation. In all 48 hours groups; tissue nitrite-nitrate concentrations were high but in the 72 hours group these values were higher than in the first group. In the 80 hours group; concentrations were lower and were statistically significant when compared to the 48 and 72 hours groups. Discussion: NO seems to be effective in early phases of vasculogenesis and angiogenesis; but its effect decreases with time.