Analytical Determination of the Oxazolidinone Antibiotic Linezolid at a Pencil Graphite and Carbon Paste Electrodes

Abstract

Electrochemical properties of linezolid, an antibiotic drug, using unmodified renewable pencil graphite and carbon paste electrodes, were investigated from pH 2 to 10 in Britton-Robinson buffer solution. Experimental studies were examined by square-wave (SW) and cyclic voltammetry (CV). Firstly, parameters affecting the performance of both electrode, such as electrode activation, support electrolyte selection, pH effect, interval of potentials, scan rates and voltammetric method parameters were investigated in detail by using square wave voltammetry. Linezolid was not reversibly oxidized in supporting electrolytes solutions, yielding well-defined peaks in the oxidation potential interval. Using the pencil graphite electrode (PGE), a linearity range between peak current and concentration was obtained between 0.01 and 0.2 mu g mL(-1) (2.96x10(-8)-5.93x10(-7) M), other one, at carbon paste electrode (CPE), a linearity range between 0.1 and 7.5 mu g mL(-1) (2.96x10(-7)-2.22x10(-5) M) was obtained by SWV. The selectivity, sensitivity, precision, and other validation parameters of the developed voltammetric method was evaluated. Lastly, the concentration of linezolid was examined in pharmaceutical and biological samples with standard addition technique.