Electrochemical Characterization of Vitamin B2 (Riboflavin) at Ag Nanoparticles Synthesized by Green Chemistry Dispersed on Reduced Graphene Oxide

Abstract

The present study describes a novel electrochemical riboflavin (RF) sensor based on the green synthesis of silver (Ag) nanoparticles dispersed on reduced graphene oxide (rGO). The Ag nanoparticles was synthesized using Arum italicum leaf extract as reducing and stabilizing agent. The characterization of Ag nanoparticles was carried out using FT-IR, XRD, and SEM-EDX. The electrochemical studies were performed using cyclic voltammetry and differential pulse voltammetry. The Ag/rGO modified glassy carbon electrode (Ag/rGO/GCE) exhibited excellent electrochemical responses to the oxidation/reduction (redox) of RF. The sensor exhibited a linear determination range from 2 x 10-9 to 2.2 x 10-6 M with limit of detection (LOD) of 0.6 nM. The sensor had the sensitivity of 71.4 mu A mu M-1 cm-2. The sensor had satisfactory selectivity, reproducibility of 3.4 % and storage stability of 90.4 %. Also, the fabricated sensor was proved to be useful for the determination of RF in pharmaceutical samples.