Electrochemical Determination of Norepinephrine by Adsorptive Stripping Voltammetry Using a Bare Boron-Doped Diamond Electrode

Abstract

A boron-doped diamond electrode was used for the first time for sensitive and selective analysis of norepinephrine (NE) using square wave voltammetry (SWV) technique. NE displayed one well-defined, irreversible and adsorption-controlled oxidation peak at about +0.85 V (vs. Ag/AgCl) in Britton-Robinson buffer (BR, 0.1 M, pH 2.0) using cyclic voltammetry (CV) technique. The effect of supporting electrolyte, solution pH and instrumental variables on oxidation peak current were optimized. In optimal experiment conditions, it was found that there was an excellent correlation between oxidation peak current and NE concentration in the range of 1 to 100 μg mL-1 (4.9×10-6 M-4.9×10-4 M) with a detection limit of 0.254 μg mL-1 (1.2×10-6 M) in 0.1 M BR buffer (pH 2.0) solution employing square-wave stripping mode. The developed technique can be used for the quantification of NE in the pharmaceutical formulation with acceptable recoveries. © 2020 Elsevier B.V., All rights reserved.