Morfological and Histometric Studies on Mast Cell Distribution and Heterogeneity, Present in the Lower Respiratory Tract and in the Lung of Local Duck (Anas Platyrhnchase) and Goose (Anser Anser)

Abstract

This study was performed to determine mast cell distribution and heterogeneity present in the lower respiratory tract and in the lung of duck and goose. In this study, 6 ducks and 6 goose were used. Sufficient amount of tissue pieces from trachea, syrinks, bronches and lungs were taken from the choosen poultries. The samples were fixed in BLA (Basic Lead Acetate - Mota), Carnoy, IFAA (Isotonic Formaldehyde Acetic Acid), then, after routin tissue follow up, the samples were blocked with paraplast. Obtained sections (6-7 mu m) were stained with 0.5% toluidine blue and alcian blue - safranin O (AB/SO). Sections stained with toloudine blue were examined under microscope (40 objective) and the number of mast cell in 1 mm(2) were determined. In both animal species; number of mast cells were changable according to fixation technique, with the highest number in lung samples. For numerical results; the most appropriate fixation technique was IFAA and BLA respectively. In AB/SO staining in trachea, syrinks bronches and lungs of geese, mast cells with SO (+), AB (+) and mix granules were obserwed. On the other hand; with the same staining in ducks; AB (+) cells in trachea, AB (+), SO (+) and mix charecteristic mast cells in syrinks, bronch and lungs, were obserwed.