Effect of Anakinra and Infliximab on Oxidative Stress and Caspase Activation in Ptz-Induced Acute Seizure in Rats

Abstract

We aimed to examine the effects of Anakinra (AKR) and Infliximab (IFB) on increased oxidative stress (OS) and proapoptotic pathway parameters after an epileptic attack. The rats were divided into four groups; control, pentylenetetrazol (PTZ), PTZ+Anakinra, and PTZ+Infliximab groups. AKR and IFB treatment was done 30 min. after PTZ administration, and rats were sacrificed after 24 h. The acute epilepsy model we created with PTZ in the rats and seizure symptoms were evaluated using the Racine classification and the delay time to the first myoclonic jerk. We also examined the levels of tota l antioxidant status (TAS), total oxidative stress (TOS), neurotrophin brain-derived neurotrophic factor (BDNF), caspase 3 and caspase 9 in the cortex and hippocampus. Post-treatment of AKR and IFB decreased PTZ-induced OS in the cortex and hippocampus while TAS levels also increased. In addition, it was observed that AKR and IFB decreased the levels of BDNF, caspase 3 and caspase 9 compared to the PTZ group. These findings showed that AKR and IFB might be used as important therapeutic agents to inhibit OS and apoptosis mechanisms that may occur after PTZ-induced epileptic attacks. © 2023, Yuzuncu Yil Universitesi Tip Fakultesi. All rights reserved.