The Effects of Some Pesticides on Human and Bovine Erythrocyte Carbonic Anhydrase Enzyme Activities in Vitro

Abstract

The influence of Penncozeb [Mangan-zinc ethylenediamine-bisdithicarbamate], Dithane, [Manganese etylenebisdithiocarbamate], Cupravit [Cuper oksichloride]. Bayleton [1-(4- chlorophenoxy)-3.3-dimethyl-1-(1H-1.2.4-Thiazol-1-yl)-2.2-butanon], Baythroid [Cyano-(4 fluoro-3-phenoxyphenyl)-methyl-3-(2.2-dichloroethyl -2. 2 metyl - cyclopropancarboxylate], Mavrik [-cyano-3-phenoxybenzyl N-(2-chloro-trifluoro - p -tolyl], Talstar [2-methyl biphenyl-3-ylmethyl(z)-(IRS,3RS)-3-(2-chloro-3.3.3 -triflouroprop -1-enyl)=Dimethylcyclo propenecarboxyate], Endosulfan [6.7.8.9.10.10-hekzachloro-1.5.5a.6.9.9a - hekzahydro-6. 9-methano-2.3.4 - benzole dioxi atehiepin 3-oxide] has been investigated on human erythrocyt carbonic anhydrase isozymes (HCA-I, HCA-II) and bovine erythrocyte carbonic anhydrase (BCA) in vitro. Among the chemicals; Baythroid, Talstar, Mavrik were determined to have inhibition effect, on bovine CA and human CA isoenzymes. Dithane had effect of inhibition only on BCA. The I50 values of chemicals caused inhibition were determined by means of activity percentage [I] diagrams. The values were 2.77×10-3 M. 3.84×10-3 M, 1.56×10-2M, 2.36×10-2 M for BCA, respectively. The values of same chemicals except dithane were 1.67×10-3 M, 4.64×10-3 M, 3.9×10-3 M for HCA-I, and 3.16×10-2 M, 2.81×10-3 M, 1.04×10-2 M for 1HCA-II, respectively. On the other hand, Changes occurred in stock concentration of chemicals showed activator effect were calculated. According to the results reached, 10-2 M cupravit, 4.17×10-2 M endosulfan and 10-2 M bayleton in stock concentration activated on both BCA and HCA-II at different levels. The values activation increases of these chemicals on BCA were 29.90, 46.40, 43.70 %, and 67.11, 57.23, 31.88 % for HCA-II, respectively. Cupravit and bayleton which activated to BCA and HCA-II were ineffective on HCA-I. Values of endosulfan was determined to be 114.40 %. Penncozeb was recorded ineffective for all isozymes. Copyright © 1996 by Marcel Dekker, Inc.