Protective Effect of Erythropoietin on Ischemia-Reperfusion Model in Rat Ovary

Abstract

Objective: In this study, the purpose was to investigate the effects of erythropoietin (EPO) on ischemia-reperfusion model that was made by experimental ovarian torsion- detorsion. Material and Methods: Twenty five Wister albino type rats were used in the study. They were randomly divided into three groups. Ten rats consisted the group that received no erythropoietin after the detorsion performed on 24th hour after ovarian torsion (group 1); 10 rats consisted the group that received intraperitoneal EPO after the detorsion that was performed at the 24th hour of ovarian torsion (group 2) and five rats consisted the sham group (group 3). After the surgical operation, right ovaries of all groups were excised on 7th day and blood samples were taken. Histopathologic findings and blood parameters of three groups were compared. Statistical analyses were made by SPSS package. One way ANOVA and t-test for independent samples were used. Results: Histopathologic findings of the group that received EPO were statistically different from the group that did not received EPO. In the group that did not receive EPO, tissue integrity deteriorated with partial necrosis and in some, there was near to total necrosis. In the group that received EPO, no necrosis was seen. After the treatment in the EPO administered group, the blood hemoglobin, hematocrit and red blood cell count were high and the differences were statistically significant. Conclusion: In ischemia-reperfusion model that was performed by ovarian torsion-detorsion, the histopathologic findings of the group that received EPO were different from the other groups, and the difference was statistically significant. In the cases of surgical detorsion that was made after ovarian torsion, intraperitoneal EPO injection had positive effects on tissue life.