Electrooxidation of Tetracycline Antibiotic Demeclocycline at Unmodified Boron-Doped Diamond Electrode and Its Enhancement Determination in Surfactant-Containing Media

Abstract

In this paper, for the first time, the study of voltammetric determination of tetracycline antibiotic demeclocycline was conducted. The oxidation of compound was investigated using a commercially available boron-doped diamond electrode pretreated electrochemically (anodic and subsequent cathodic). Addition of anionic surfactant, sodium dodecylsulfate (SDS) and cationic surfactant, cetyltrimethylammonium bromide (CTAB) to the demeclocycline-containing electrolyte solution at pH 2.0 and 9.0, respectively, was found to improve the sensitivity of the stripping voltammetric measurements. Employing square-wave stripping mode (after 30 s accumulation at open-circuit condition) in Britton-Robinson buffer, the limits of detection were found to be 1.17 mu g mL(-1) (2.3 x 10(-6) M) for 4 x 10(-4) SDS-containing buffer solution at pH 2, and 0.24 mu g mL(-1) (4.8 x 10(-7) M) for 1 x 10(-4) CTAB-containing buffer solution at pH 9.0. The feasibility of the developed approach for the quantification of demeclocycline was tested in urine samples.