Electrochemical Behavior of Janus Kinase Inhibitor Ruxolitinib at a Taurine-Electropolymerized Carbon Paste Electrode: Insights Into Sensing Mechanisms

Abstract

Ruxolitinib (RXL) is a Janus kinase inhibitor used for treating intermediate- or high-risk myelofibrosis. This study presents an electrode modified with electrochemically polymerized taurine on a carbon paste electrode via cyclic voltammetry (CV). The surface characterization of the poly(taurine)-CP electrode was evaluated by using electrochemical (electrochemical impedance spectroscopy-EIS, CV), morphological (scanning electron microscope-SEM), and spectroscopic (Fourier-transform infrared spectroscopy-FT-IR) techniques. Under optimized conditions, RXL exhibited good linearity within the 0.01-1.0 mu M concentration range, with a limit of detection (LOD) of 0.005 mu M. The proposed electrochemical sensor demonstrated excellent selectivity, accuracy, precision, and repeatability. Furthermore, it effectively detected RXL in human urine and pharmaceutical samples.