Communication Between Dna Polymerases and Replication Protein a Within the Archaeal Replisome
| dc.authorid | England, Patrick/0000-0001-6410-5918 | |
| dc.authorid | Dizkirici Tekpinar, Ayten/0000-0002-0578-5092 | |
| dc.authorid | Madru, Clement/0000-0002-5412-8822 | |
| dc.authorid | Haouz, Ahmed/0000-0003-1196-1635 | |
| dc.authorid | Legrand, Pierre/0000-0003-2431-2255 | |
| dc.authorid | Martinez-Carranza, Markel/0000-0003-0192-9762 | |
| dc.authorscopusid | 57195297187 | |
| dc.authorscopusid | 58810239500 | |
| dc.authorscopusid | 56720335200 | |
| dc.authorscopusid | 7003284412 | |
| dc.authorscopusid | 57190257353 | |
| dc.authorscopusid | 6601999522 | |
| dc.authorscopusid | 6603424968 | |
| dc.authorwosid | Guijarro, J./E-6438-2019 | |
| dc.authorwosid | Legrand, Pierre/G-7709-2011 | |
| dc.contributor.author | Martinez Carranza, Markel | |
| dc.contributor.author | Vialle, Lea | |
| dc.contributor.author | Madru, Clement | |
| dc.contributor.author | Cordier, Florence | |
| dc.contributor.author | Tekpinar, Ayten Dizkirici | |
| dc.contributor.author | Haouz, Ahmed | |
| dc.contributor.author | Sauguet, Ludovic | |
| dc.date.accessioned | 2025-05-10T17:24:57Z | |
| dc.date.available | 2025-05-10T17:24:57Z | |
| dc.date.issued | 2024 | |
| dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
| dc.department-temp | [Martinez-Carranza, Markel; Madru, Clement; Tekpinar, Ayten Dizkirici; Sauguet, Ludovic] Univ Paris Cite, Inst Pasteur, Architecture & Dynam Biol Macromol, CNRS,UMR 3528, Paris, France; [Vialle, Lea; Dulermo, Remi; Henneke, Ghislaine] Univ Brest, Ifremer, CNRS, Biol & Ecol Ecoystemes Marins Profonds BEEP, Plouzane, France; [Cordier, Florence; Le Meur, Remy A.; Guijarro, J. Inaki] Univ Paris Cite, Inst Pasteur, CNRS, UMR 3528,Biol NMR & HDX-MS Technol Platform, Paris, France; [Cordier, Florence] Univ Paris Cite, Inst Pasteur, Struct Bioinformat, CNRS,UMR 3528, Paris, France; [Haouz, Ahmed] Univ Paris Cite, CNRS, Inst Pasteur, Crystallog Platform,C2RT,UMR 3528, Paris, France; [Legrand, Pierre] HelioBio Grp, Synchrotron SOLEIL, Lorme Merisiers, Saint Aubin, France; [England, Patrick] Univ Paris Cite, CNRS, Inst Pasteur, Mol Biophys Platform,C2RT,UMR 3528, Paris, France; [Tekpinar, Ayten Dizkirici] Van Yuzuncu Yil Univ, Dept Mol Biol & Genet, Van, Turkiye | en_US |
| dc.description | England, Patrick/0000-0001-6410-5918; Dizkirici Tekpinar, Ayten/0000-0002-0578-5092; Madru, Clement/0000-0002-5412-8822; Haouz, Ahmed/0000-0003-1196-1635; Legrand, Pierre/0000-0003-2431-2255; Martinez-Carranza, Markel/0000-0003-0192-9762 | en_US |
| dc.description.abstract | Replication Protein A (RPA) plays a pivotal role in DNA replication by coating and protecting exposed single-stranded DNA, and acting as a molecular hub that recruits additional replication factors. We demonstrate that archaeal RPA hosts a winged-helix domain (WH) that interacts with two key actors of the replisome: the DNA primase (PriSL) and the replicative DNA polymerase (PolD). Using an integrative structural biology approach, combining nuclear magnetic resonance, X-ray crystallography and cryo-electron microscopy, we unveil how RPA interacts with PriSL and PolD through two distinct surfaces of the WH domain: an evolutionarily conserved interface and a novel binding site. Finally, RPA is shown to stimulate the activity of PriSL in a WH-dependent manner. This study provides a molecular understanding of the WH-mediated regulatory activity in central replication factors such as RPA, which regulate genome maintenance in Archaea and Eukaryotes. | en_US |
| dc.description.sponsorship | Agence Nationale de la Recherche (French National Research Agency); ANR - postdoctoral FRM fellowship (Fondation pour la Recherche Mdicale) [060208/Z/00/Z, 093305/Z/10/Z, PID: 21658]; Welcome JIF award [4014526]; Rgion Ile de France (SESAME) | en_US |
| dc.description.sponsorship | We gratefully acknowledge the financial support of ANR (Grant ARCHAPRIM). C.M. was funded by a postdoctoral Pasteur-Roux-Cantarini fellowship. M.M.C. was funded by a postdoctoral FRM fellowship (Fondation pour la Recherche Medicale). We wish to acknowledge SOLEIL for the provision of synchrotron radiation facilities and the staff of beamlines PROXIMA-1 and PROXIMA-2 (Saint-Aubin, France). We would like to thank the staff of the Nanoimaging Core Facility at Institut Pasteur for cryo-EM instrument access for the preliminary cryo-EM datasets. Electron microscopy instrument time was provided through the OPIC electron microscopy facility, a UK Instruct-ERIC Centre, which was founded by a Welcome JIF award (060208/Z/00/Z) and is supported by a Welcome equipment grant (093305/Z/10/Z). Cryo-EM final data collection experiments were funded by UK Instruct-ERIC, PID: 21658. The 800-MHz NMR spectrometer of the Institut Pasteur was partially funded by the Region Ile de France (SESAME 2014 NMRCHR grant no 4014526). | en_US |
| dc.description.woscitationindex | Science Citation Index Expanded | |
| dc.identifier.doi | 10.1038/s41467-024-55365-w | |
| dc.identifier.issn | 2041-1723 | |
| dc.identifier.issue | 1 | en_US |
| dc.identifier.pmid | 39738083 | |
| dc.identifier.scopus | 2-s2.0-85213685198 | |
| dc.identifier.scopusquality | Q1 | |
| dc.identifier.uri | https://doi.org/10.1038/s41467-024-55365-w | |
| dc.identifier.uri | https://hdl.handle.net/20.500.14720/11220 | |
| dc.identifier.volume | 15 | en_US |
| dc.identifier.wos | WOS:001386373100045 | |
| dc.identifier.wosquality | Q1 | |
| dc.language.iso | en | en_US |
| dc.publisher | Nature Portfolio | en_US |
| dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.title | Communication Between Dna Polymerases and Replication Protein a Within the Archaeal Replisome | en_US |
| dc.type | Article | en_US |