Sedative and Anxiolytic Activities of Opuntia Ficus Indica (L.) Mill.: an Experimental Assessment in Mice
dc.authorid | Sobarzo-Sanchez, Eduardo/0000-0002-2790-2544 | |
dc.authorid | Kupeli Akkol, Esra/0000-0002-5829-7869 | |
dc.authorid | Karpuz Agoren, Busra/0000-0001-6095-5210 | |
dc.authorid | Ilhan, Mert/0000-0001-5042-3915 | |
dc.authorscopusid | 23096992900 | |
dc.authorscopusid | 56020070400 | |
dc.authorscopusid | 57210468922 | |
dc.authorscopusid | 45261107000 | |
dc.authorscopusid | 6601935879 | |
dc.authorwosid | Akkol, Esra/Abi-4678-2020 | |
dc.authorwosid | Karpuz Ağören, Büşra/Jjd-7643-2023 | |
dc.authorwosid | Ilhan, Mert/Hlh-6199-2023 | |
dc.authorwosid | Sobarzo-Sánchez, Eduardo/B-1507-2012 | |
dc.contributor.author | Akkol, Esra Kupeli | |
dc.contributor.author | Ilhan, Mert | |
dc.contributor.author | Karpuz, Busra | |
dc.contributor.author | Genc, Yasin | |
dc.contributor.author | Sobarzo-Sanchez, Eduardo | |
dc.date.accessioned | 2025-05-10T17:04:31Z | |
dc.date.available | 2025-05-10T17:04:31Z | |
dc.date.issued | 2020 | |
dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
dc.department-temp | [Akkol, Esra Kupeli; Karpuz, Busra] Gazi Univ, Fac Pharm, Dept Pharmacognosy, TR-06330 Ankara, Turkey; [Ilhan, Mert] Van Yuzuncu Yil Univ, Fac Pharm, Dept Pharmacognosy, TR-65080 Tusba, Van, Turkey; [Genc, Yasin] Hacettepe Univ, Fac Pharm, Dept Pharmacognosy, TR-06100 Ankara, Turkey; [Sobarzo-Sanchez, Eduardo] Univ Cent Chile, Fac Ciencias Salud, Inst Invest & Innovac Salud, Santiago 8330507, Chile; [Sobarzo-Sanchez, Eduardo] Univ Santiago de Compostela, Fac Pharm, Dept Organ Chem, Santiago De Compostela 15782, Spain | en_US |
dc.description | Sobarzo-Sanchez, Eduardo/0000-0002-2790-2544; Kupeli Akkol, Esra/0000-0002-5829-7869; Karpuz Agoren, Busra/0000-0001-6095-5210; Ilhan, Mert/0000-0001-5042-3915 | en_US |
dc.description.abstract | Ethnobotanical field surveys revealed the use of fruits of Opuntia ficus indica (L.) Mill. for treating diabetes, burns, bronchial asthma, constipation, kidney stones, and rheumatic pains and as a sedative in Turkish folk medicine. This study aimed to verify the efficacy of the fruits of O. ficus indica experimentally and to define components responsible for the activity using bioassay-guided procedures. The crude methanolic extract of the fruits was sequentially fractionated into five subextracts: n-hexane, dichloromethane, ethyl acetate, n-butanol, and water. Further experiments were carried out on the most active subextract, that is, the ethyl acetate (EtOAc) subextract, which was further subjected to fractionation through successive column chromatographic applications on Sephadex LH-20. For activity assessment, each extract or fraction was submitted to bioassay systems; traction test, fireplace test, hole-board test, elevated plus-maze test, and open-field test were used for sedative and anxiolytic effects, and a thiopental-induced sleeping test was used for the hypnotic effect. Among the subextracts obtained from the methanolic extract, the EtOAc subextract showed significant sedative and anxiolytic effects in the bioassay systems. From the EtOAc subextract, major components were isolated, and their structures were determined as isorhamnetin, isorhamnetin 3-O-glucoside, isorhamnetin 3-O-rutinoside, and kaempferol 3-O-rutinoside using spectral techniques. In conclusion, this study confirmed the claimed use of the plant against anxiety in Turkish folk medicine. | en_US |
dc.description.woscitationindex | Science Citation Index Expanded | |
dc.identifier.doi | 10.3390/molecules25081844 | |
dc.identifier.issn | 1420-3049 | |
dc.identifier.issue | 8 | en_US |
dc.identifier.pmid | 32316321 | |
dc.identifier.scopus | 2-s2.0-85083570525 | |
dc.identifier.scopusquality | Q2 | |
dc.identifier.uri | https://doi.org/10.3390/molecules25081844 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14720/6017 | |
dc.identifier.volume | 25 | en_US |
dc.identifier.wos | WOS:000534617500036 | |
dc.identifier.wosquality | Q2 | |
dc.language.iso | en | en_US |
dc.publisher | Mdpi | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | Anxiolytic | en_US |
dc.subject | Cactaceae | en_US |
dc.subject | Hypnotic | en_US |
dc.subject | Mice | en_US |
dc.subject | Opuntia Ficus Indica | en_US |
dc.subject | Sedative | en_US |
dc.title | Sedative and Anxiolytic Activities of Opuntia Ficus Indica (L.) Mill.: an Experimental Assessment in Mice | en_US |
dc.type | Article | en_US |