Differential Activation and Expression of Insulin Receptor Substrate 1 (Irs1) in Mononuclear Cells of Type 2 Diabetes Patients After Insulin Stimulation
dc.authorid | Balci, Mustafa Kemal/0000-0002-6494-3249 | |
dc.authorid | Ozdem, Sadi/0000-0003-4325-5209 | |
dc.authorid | Ozel, Deniz/0000-0001-9221-5452 | |
dc.authorscopusid | 55910152400 | |
dc.authorscopusid | 57210688369 | |
dc.authorscopusid | 58312453700 | |
dc.authorscopusid | 57191881326 | |
dc.authorscopusid | 6701608054 | |
dc.authorscopusid | 36060045200 | |
dc.authorscopusid | 36060045200 | |
dc.authorwosid | Özel, Deniz/Aax-6644-2021 | |
dc.authorwosid | Balci, Mustafa Kemal/C-2979-2016 | |
dc.authorwosid | Ozdem, Sadi/C-1140-2016 | |
dc.contributor.author | Gorgisen, G. | |
dc.contributor.author | Balci, M.K. | |
dc.contributor.author | Celik, F.C. | |
dc.contributor.author | Gokkaya, M. | |
dc.contributor.author | Ozdem, S. | |
dc.contributor.author | Ozel, D. | |
dc.contributor.author | Ozes, O.N. | |
dc.date.accessioned | 2025-05-10T17:00:51Z | |
dc.date.available | 2025-05-10T17:00:51Z | |
dc.date.issued | 2016 | |
dc.department | T.C. Van Yüzüncü Yıl Üniversitesi | en_US |
dc.department-temp | Gorgisen G., Akdeniz University Medical School, Department of Medical Biology and Genetics, Antalya, Turkey, Yuzuncu Yil University Medical School, Department of Medical Biology, Van, Turkey; Balci M.K., Akdeniz University Medical School, Department of Endocrinology, Antalya, Turkey; Celik F.C., Akdeniz University Medical School, Department of Endocrinology, Antalya, Turkey; Gokkaya M., Akdeniz University Medical School, Department of Endocrinology, Antalya, Turkey; Ozdem S., Akdeniz University Medical School, Department of Pharmacology, Antalya, Turkey; Ozel D., Akdeniz University Medical School, Department of Biostatistics, Antalya, Turkey; Ozes O.N., Akdeniz University Medical School, Department of Medical Biology and Genetics, Antalya, Turkey | en_US |
dc.description.abstract | Insulin regulates the glucose homeostasis by inducing tyrosine phosphorylation of insulin receptor substrate (IRS) proteins. IRS1 is the best studied member of this family and insulin-induced Tyrosine phosphorylation of (YXXM) motifs provides docking site for SH2 domain-containing proteins. Recent studies have suggested that genetic and/or environmental factors may affect the expression and phosphorylation levels of IRS1, and these could be important for development of insulin resistance. To shed light to the molecular basis of type 2 diabetes we wanted to determine whether YXXM motifs are genetically modified in these patients. We have isolated mononuclear cells of eighteen type 2 diabetes patients and prepared genomic DNA and protein lysates from these cells. The genomic DNA was used to sequence IRS1 gene, and protein lysates were used to determine the expression and phosphotyrosine levels of IRS1 after insulin stimulation. Although, we did not detect any mutations at/or near the YXXM coding regions in patients' DNA, immunprecipitation analysis of IRS1 indicated decreased levels of expression and tyrosine phosphorylation of IRS1 in patient's samples compared to that of healthy controls. Our results suggest that mononuclear cells of patients can be used to test the levels of insulin responsiveness before therapy. © 2016 by the C.M.B. Association. | en_US |
dc.description.sponsorship | Istanbul Üniversitesi, (25662); Istanbul Üniversitesi | en_US |
dc.description.woscitationindex | Science Citation Index Expanded | |
dc.identifier.doi | 10.14715/cmb/2016.62.2.5 | |
dc.identifier.endpage | 30 | en_US |
dc.identifier.issn | 0145-5680 | |
dc.identifier.issue | 3 | en_US |
dc.identifier.pmid | 26950447 | |
dc.identifier.pmid | 26950447 | |
dc.identifier.scopus | 2-s2.0-84994462132 | |
dc.identifier.scopusquality | Q4 | |
dc.identifier.startpage | 25 | en_US |
dc.identifier.uri | https://doi.org/10.14715/cmb/2016.62.2.5 | |
dc.identifier.uri | https://hdl.handle.net/20.500.14720/4978 | |
dc.identifier.volume | 62 | en_US |
dc.identifier.wos | WOS:000371286100005 | |
dc.identifier.wosquality | Q4 | |
dc.language.iso | en | en_US |
dc.publisher | Cellular and Molecular Biology Association | en_US |
dc.relation.ispartof | Cellular and Molecular Biology | en_US |
dc.relation.publicationcategory | Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı | en_US |
dc.rights | info:eu-repo/semantics/closedAccess | en_US |
dc.subject | Diabetes Mellitus | en_US |
dc.subject | Insulin Receptor Substrate Protein | en_US |
dc.subject | Insulin Resistance | en_US |
dc.subject | Insulin Responsiveness | en_US |
dc.subject | Type 2 | en_US |
dc.title | Differential Activation and Expression of Insulin Receptor Substrate 1 (Irs1) in Mononuclear Cells of Type 2 Diabetes Patients After Insulin Stimulation | en_US |
dc.type | Article | en_US |