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Differential Activation and Expression of Insulin Receptor Substrate 1 (Irs1) in Mononuclear Cells of Type 2 Diabetes Patients After Insulin Stimulation

dc.authorid Balci, Mustafa Kemal/0000-0002-6494-3249
dc.authorid Ozdem, Sadi/0000-0003-4325-5209
dc.authorid Ozel, Deniz/0000-0001-9221-5452
dc.authorscopusid 55910152400
dc.authorscopusid 57210688369
dc.authorscopusid 58312453700
dc.authorscopusid 57191881326
dc.authorscopusid 6701608054
dc.authorscopusid 36060045200
dc.authorscopusid 36060045200
dc.authorwosid Özel, Deniz/Aax-6644-2021
dc.authorwosid Balci, Mustafa Kemal/C-2979-2016
dc.authorwosid Ozdem, Sadi/C-1140-2016
dc.contributor.author Gorgisen, G.
dc.contributor.author Balci, M.K.
dc.contributor.author Celik, F.C.
dc.contributor.author Gokkaya, M.
dc.contributor.author Ozdem, S.
dc.contributor.author Ozel, D.
dc.contributor.author Ozes, O.N.
dc.date.accessioned 2025-05-10T17:00:51Z
dc.date.available 2025-05-10T17:00:51Z
dc.date.issued 2016
dc.department T.C. Van Yüzüncü Yıl Üniversitesi en_US
dc.department-temp Gorgisen G., Akdeniz University Medical School, Department of Medical Biology and Genetics, Antalya, Turkey, Yuzuncu Yil University Medical School, Department of Medical Biology, Van, Turkey; Balci M.K., Akdeniz University Medical School, Department of Endocrinology, Antalya, Turkey; Celik F.C., Akdeniz University Medical School, Department of Endocrinology, Antalya, Turkey; Gokkaya M., Akdeniz University Medical School, Department of Endocrinology, Antalya, Turkey; Ozdem S., Akdeniz University Medical School, Department of Pharmacology, Antalya, Turkey; Ozel D., Akdeniz University Medical School, Department of Biostatistics, Antalya, Turkey; Ozes O.N., Akdeniz University Medical School, Department of Medical Biology and Genetics, Antalya, Turkey en_US
dc.description.abstract Insulin regulates the glucose homeostasis by inducing tyrosine phosphorylation of insulin receptor substrate (IRS) proteins. IRS1 is the best studied member of this family and insulin-induced Tyrosine phosphorylation of (YXXM) motifs provides docking site for SH2 domain-containing proteins. Recent studies have suggested that genetic and/or environmental factors may affect the expression and phosphorylation levels of IRS1, and these could be important for development of insulin resistance. To shed light to the molecular basis of type 2 diabetes we wanted to determine whether YXXM motifs are genetically modified in these patients. We have isolated mononuclear cells of eighteen type 2 diabetes patients and prepared genomic DNA and protein lysates from these cells. The genomic DNA was used to sequence IRS1 gene, and protein lysates were used to determine the expression and phosphotyrosine levels of IRS1 after insulin stimulation. Although, we did not detect any mutations at/or near the YXXM coding regions in patients' DNA, immunprecipitation analysis of IRS1 indicated decreased levels of expression and tyrosine phosphorylation of IRS1 in patient's samples compared to that of healthy controls. Our results suggest that mononuclear cells of patients can be used to test the levels of insulin responsiveness before therapy. © 2016 by the C.M.B. Association. en_US
dc.description.sponsorship Istanbul Üniversitesi, (25662); Istanbul Üniversitesi en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.doi 10.14715/cmb/2016.62.2.5
dc.identifier.endpage 30 en_US
dc.identifier.issn 0145-5680
dc.identifier.issue 3 en_US
dc.identifier.pmid 26950447
dc.identifier.pmid 26950447
dc.identifier.scopus 2-s2.0-84994462132
dc.identifier.scopusquality Q4
dc.identifier.startpage 25 en_US
dc.identifier.uri https://doi.org/10.14715/cmb/2016.62.2.5
dc.identifier.uri https://hdl.handle.net/20.500.14720/4978
dc.identifier.volume 62 en_US
dc.identifier.wos WOS:000371286100005
dc.identifier.wosquality Q4
dc.language.iso en en_US
dc.publisher Cellular and Molecular Biology Association en_US
dc.relation.ispartof Cellular and Molecular Biology en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.subject Diabetes Mellitus en_US
dc.subject Insulin Receptor Substrate Protein en_US
dc.subject Insulin Resistance en_US
dc.subject Insulin Responsiveness en_US
dc.subject Type 2 en_US
dc.title Differential Activation and Expression of Insulin Receptor Substrate 1 (Irs1) in Mononuclear Cells of Type 2 Diabetes Patients After Insulin Stimulation en_US
dc.type Article en_US

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