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Genotyping of Brucella Isolates From Animals and Humans by Multiple-Locus Variable-Number Tandem Repeat Analysis (Mlva)

dc.authorid Nuhay, Cagatay/0000-0002-1475-3041
dc.authorid Iba Yilmaz, Sibel/0000-0002-4123-0828
dc.authorid Akar, Kadir/0000-0003-0894-7357
dc.authorscopusid 56519460700
dc.authorscopusid 56720294600
dc.authorscopusid 57208342678
dc.authorscopusid 57219482987
dc.authorscopusid 14038148900
dc.authorscopusid 54787844400
dc.authorscopusid 57204422452
dc.authorwosid Aslan, Mehtap/Aak-4873-2021
dc.authorwosid Özgen, Ediz/Aib-8466-2022
dc.authorwosid Deni̇z, İbrahim/Jvm-8731-2024
dc.authorwosid Akar, Kadir/Ahe-5056-2022
dc.authorwosid Saytekin, Ahmet Murat/Abh-1367-2020
dc.contributor.author Ozmen, Murat
dc.contributor.author Ozgen, Ediz Kagan
dc.contributor.author Sayi, Orbay
dc.contributor.author Putur, Elif Karadeniz
dc.contributor.author Okumus, Biray
dc.contributor.author Yilmaz, Sibel Iba
dc.contributor.author Akar, Kadir
dc.date.accessioned 2025-05-10T16:46:04Z
dc.date.available 2025-05-10T16:46:04Z
dc.date.issued 2023
dc.department T.C. Van Yüzüncü Yıl Üniversitesi en_US
dc.department-temp [Ozmen, Murat; Ozgen, Ediz Kagan; Sayi, Orbay; Putur, Elif Karadeniz; Okumus, Biray; Bagatir, Perihan Serifoglu] Republ Turkiye Minist Agr & Forestry, Vet Control Inst, Erzurum, Turkiye; [Yilmaz, Sibel Iba] Hlth Sci Univ, Erzurum Reg Educ & Res Hosp, Dept Infect Dis & Clin Microbiol, Erzurum, Turkiye; [Aslan, Mehtap Hulya] Hlth Sci Univ, Erzurum Reg Educ & Res Hosp, Depertmant Microbiol, Erzurum, Turkiye; [Ulucan, Mustafa] Iskilip Dist Directorate Agr & Forestry, Corum, Turkiye; [Yanmaz, Berna] Burdur Mehmet Akif Ersoy Univ, Fac Vet Med, Dept Vet Publ Hlth, Burdur, Turkiye; [Turut, Nevin; Karahan, Sifa] Republ Turkiye Minist Agr & Forestry, Vet Control Inst, Adana, Turkiye; [Eroglu, Buket; Gulseren, Yildiz Oz] Republ Turkiye Minist Agr & Forestry, Vet Control Inst, Istanbul, Turkiye; [Kucukayan, Ugur] Republ Turkiye Minist Agr & Forestry, Vet Control Cent Res Inst, Ankara, Turkiye; [Nuhay, Cagatay; Eskiizmirliler, Seza] Republ Turkiye Minist Agr & Forestry, Vet Control Inst, Izmir, Turkiye; [Aka, Erdem S.] Minist Agr & Forestry, Vet Control Inst, Samsun, Turkiye; [Sarisahin, Asuman Soysal; Deniz, Ibrahim] Republ Turkiye Minist Agr & Forestry, Vet Control Inst, Konya, Turkiye; [Birben, Nurgul; Karakus, Kerem] Republ Turkiye Minist Agr & Forestry, Vet Control Inst, Elazig, Turkiye; [Sen, Esra] Gen Directorate Food & Control, Anim Hlth Quarantine Dept Head, Dept Brucellosis & TB, Ankara, Turkiye; [Saytekin, Ahmet Murat] Harran Univ, Fac Vet Med, Dept Microbiol, Sanliurfa, Turkiye; [Akar, Kadir] Van Yuzuncu Yil Univ, Fac Vet Med, Dept Microbiol, Van, Turkiye; [Ozgen, Ediz Kagan] Erzurum Vet Kontrol Enstitusu, Palandoken Erzurum, Turkiye en_US
dc.description Nuhay, Cagatay/0000-0002-1475-3041; Iba Yilmaz, Sibel/0000-0002-4123-0828; Akar, Kadir/0000-0003-0894-7357 en_US
dc.description.abstract This study investigates country-wide genotype variations through the genotyping of Brucella strains isolated from domestic ruminants and humans. The Brucella spp. isolated from samples taken from animals and humans were first identified as B. abortus and B. melitensis by real-time PCR, and the MLVA-16 approach was then used for the genotyping of the identified isolates. For the study, 416 Brucella spp. were isolated from aborted fetus samples examined between 2018 and 2021, and 74 Brucella spp. from infected humans. Of the 74 human isolates analyzed, 1.3% were identified as B. abortus and 98.7% (73/74) as B. melitensis. The MLVA-16 typing method revealed 30 clonal groups for B. abortus and 37 clonal groups for B. melitensis from which the dominant genotypes and similarities with human isolates in Turkiye were determined. en_US
dc.description.sponsorship Republic of Tuerkiye Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies [TAGEM/HSGYAD/UE/18/A5/P1/707] en_US
dc.description.sponsorship This study was supported by the Republic of Tuerkiye Ministry of Agriculture and Forestry, General Directorate of Agricultural Research and Policies (project number: TAGEM/HSGYAD/UE/18/A5/P1/707) . en_US
dc.description.woscitationindex Science Citation Index Expanded
dc.identifier.doi 10.1016/j.cimid.2023.101981
dc.identifier.issn 0147-9571
dc.identifier.issn 1878-1667
dc.identifier.pmid 37043846
dc.identifier.scopus 2-s2.0-85151808285
dc.identifier.scopusquality Q3
dc.identifier.uri https://doi.org/10.1016/j.cimid.2023.101981
dc.identifier.uri https://hdl.handle.net/20.500.14720/1021
dc.identifier.volume 96 en_US
dc.identifier.wos WOS:001029921800001
dc.identifier.wosquality Q2
dc.language.iso en en_US
dc.publisher Elsevier Sci Ltd en_US
dc.relation.publicationcategory Makale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanı en_US
dc.rights info:eu-repo/semantics/closedAccess en_US
dc.subject B en_US
dc.subject Melitensis en_US
dc.subject Mlva-16 en_US
dc.subject Ruminant en_US
dc.subject Turkiye en_US
dc.title Genotyping of Brucella Isolates From Animals and Humans by Multiple-Locus Variable-Number Tandem Repeat Analysis (Mlva) en_US
dc.type Article en_US

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