Evaluation of Effectiveness of Neutral-Ph Superoxidized Solution (Nsos) With Peritoneal Lavage in Rat Fecal Peritonitis Model: an Experimental Study

Abstract

This experimental study aimed to evaluate the effectiveness of NSOS (Neutral-pH superoxidized solution) with peritoneal lavage in rat fecal peritonitis models. Forty Wistar rats weighing between 250-300 g were used for the study. All rats were inducted for fecal peritonitis formation. The rats were divided into five groups as follows; Group 0: control, no intervention. Group 1: Application of 10 mg/kg NSOS into the peritoneal cavity after 6 h of induction of peritonitis. Group 2: 10 mg/kg NSOS application and repeated at 24 and 48 h. Group 3: NSOS + Antibiotic treatment (Seftriakson 30 mg/kg/ day IM 2x1 + metronidazol 15 mg/kg/day IM 2x1). Group 4: Only antibiotic treatment (Seftriakson 30 mg/kg/day IM 2x1 + metronidazol 15 mg/kg/day IM 2x1). The animals were examined for peritoneal and thoracal abscess formation, adherences, and any abnormality with inspection after sacrificing on the 7th day. The peritoneal lavage fluid culture for microbiological analysis and blood samples were taken for blood cultures, biochemical and infectious parameters of WBC, CRP, TNF- alpha, IL-6, IL-1 beta, and IL-10. Peritonitis was developed in all rats at the end of follow-up. No death was observed in rats on the seventh day of the experiment. Group 3 (NSOS + Antibiotic treatment) showed the most significant improvement in the infection of peritoneal fluid. NSOS and antibiotic together (Group 3) were found to be more effective against Klebsiella than Enterococcus sp. The blood cultures showed a significant reduction in all groups. The infectious parameters including IL-6, IL-1 beta, and IL-10 showed no significant difference in the first week of treatment between all groups. Only TNF- alpha was observed significantly lower in group 3 when compared to the other groups (P=0.001). Peritoneal lavage with neutral pH-superoxidized water plus an antibiotic regimen is the most effective treatment in the rat fecal peritonitis model. Further studies including human subjects are needed to investigate its effectiveness and validity