The Effects of 17α-Ethynylestradiol, 4-Nonylphenol and Phenol Red on Vitellogenin Synthesis in Juvenile Chalcalburnus Tarichi Primary Hepatocyte Culture

Abstract

In this study, the effects of the endocrine-disrupting chemicals known as 17 alpha-ethynylestradiol (EE2), 4-nonylphenol (NP) and phenol red on vitellogenin (Vtg) synthesis in juvenile Chalcalburnus tarichi primary hepatocyte culture were investigated depending on dose and time. Hepatocytes were isolated by collagenase perfusion method and the cells were kept alive in cell culture for more than 2 weeks without using serum. As a result, it was determined that EE2 increased the Vtg synthesis depending on dose and time (p<0.05), while NP and phenol red did not have any effect on the Vtg synthesis in the Chalcalburnus tarichi primary hepatocyte culture. Besides that, it was observed that high doses of NP (10(-3) and 10(-4) M) had toxic effects to the hepatocyte culture, too.