Effects of Dexamethasone on Trace Elements and Serum Protein Patterns Following Brain Trauma in Rats

Abstract

The effect of dexamethasone (Dxm) on trace elements and serum protein patterns was investigated in male Sprague-Dawley rats subjected to brain trauma. After 6- or 24 h of the traumatic incident, the level of serum copper was significantly higher in the Dxm-treated rats, compared to controls (p < 0.05). The corresponding levels of zinc and iron did not show significant differences. The zinc level returned to normal 24 h after trauma. After 6 and 24 h of trauma, the sodium dodecyl sulfate (SDS)-polyacrylamide gel patterns of serum proteins showed that a 41.6-kDa protein was significantly increased in the Dxm-treated animals. Two proteins weighing 26.6 kDa and 55.1 kDa did not show Dxm-induced changes. These results suggest that increases in the copper-zinc ratio and the changes of the 26.6-kDa, 41.6-kDa, and 55.1-kDa proteins might be a useful prognostic indicator for severe traumatic brain injury.