The Inhibitory Impact of Glutathione (GSH) and Ascorbic Acid (Vitamin C) Compounds on Glucose-6 Dehydrogenase (G6PD) Enzyme Purified from Sheep Liver

Abstract

Objective:Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme with many essential biochemical functions. However, in various cancer diseases, increased activity of G6PD causes cancer cells to grow, so G6PD inhibitors have become a significant area of research in cancer treatment.Materials and methods:Here, G6PD was purified 4530-fold with affinity chromatography using 2',5 '-ADP Sepharose 4B from sheep liver. The effects of reduced glutathione (GSH) and ascorbic acid (vitamin C) on G6PD activity were explored.Results and discussion:GSH and ascorbic acid showed a significant inhibitory effect on G6PD, and IC50 values were found as 0.37 mu M and 34.66 mu M, respectively. The inhibition type from Lineweaver-Burk plots of these compounds was identified as non-competitive inhibition. The Ki values of GSH and ascorbic acid were calculated as 0.48 mu M and 30.47 mu M, respectively.Conclusion:In this study, it was observed that GSH and ascorbic acid antioxidant compounds exhibit an inhibitory effect on G6PD and may be protective and preventive against cancer.