Antioxidant, Antiradical Properties, Vitamin and Trace Element Status of Artemisia Spicigera C. Koch Distributed in the Türkiye Flora

Abstract

It is known that the active substances present in plants contribute to many biochemical processes and provide the balance of organisms. This study aims to determine the antioxidant and antiradical properties of the methanol extract of Artemisia spicigera (A. spicigera) C. Koch leaves and to measure the levels of trace elements and vitamins E and K. Trace element analyses were performed by the inductively coupled plasma-optical emission spectroscopy dry ashing dry ashing and vitamin analyses were performed by the high performance liquid chromatography method. Vitamin C, total phenol and flavonoid contencts, antioxidant capacity, DPPH center dot, ABTS center dot+, O2 center dot-, H2O2 and OH center dot values were determined spectrophotometrically. Total phenolic and flavonoid contents and antioxidant capacity were determined as 18.45 +/- 0.39 mgGA/g, 26.29 +/- 1.64 mgQE/g and 44.08 +/- 2.91 mMAA/g, respectively. Mn, Zn, Cu, alpha-tocopherol, phylloquinone and ascorbic acid levels were found as 287.32 +/- 0.57, 104.36 +/- 2.12, 37.40 +/- 0.10, 10.28 +/- 0.75, 0.149 +/- 0.047 mu mol/kg and 217.36 +/- 29.20 mg/100g, respectively. In vitro antioxidant properties determined by DPPH center dot, ABTS center dot+, H2O2 and OH center dot assays showed maximum inhibitory with respective IC50 values of 61.83 +/- 0.68, 71.74 +/- 0.79, 34.25 +/- 0.74 and 77.91 +/- 0.88 mu g/mL. The IC50 values of these assays showed promising antioxidant power. The results obtained in this study showed that the plant leaves migth be a potential candidate for in vivo studies to be evaluated in the future. A. spicigera can be also used as a potential supplement or in the pharmaceutical industries as a source of natural antioxidants.